The aim of this research was to determine the impact of biofilms on water distribution system of FederalUniversity Birnin Kebbi. Samples were collected from different water distribution pipelines in the University.Total heterotrophic count was carried out to determine the microbial load and Most Probable Method (MPN)was used to detect the presence of fecal coliforms in the water. Total heterotrophic bacterial count rangedbetween 1.6×103 to 3.9×103 cfu/ml. Escherichia Coli showed the highest frequency (25%) of occurrence, whilethe least frequency of occurrence (5%) was recorded for Klebsiella spp and Enterobacter spp respectively. Theisolates identified were Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella spp,Enterobacteria Spp, Salmonella Spp and Bacillus Spp. Presence of these isolates is of significant concern andmay cause some water borne diseases like diarrhea, dysentery etc. It is therefore recommended that water boardtreatment plant should use disinfectant chemicals like chlorine and perform regular proactive preventivemaintenance, microbial monitoring and infrastructure replacement and repair so as to reduce the occurrence ofbiofilms in the Water Distribution system.