No abstract

Corona viruses are a family of viruses that can cause diseases such as the common cold and acute respiratory infection and in 2019, a new type of corona virus was discovered that caused an outbreak of a disease that originated in China. The virus is known as severe acute respiratory syndrome corona virus (SARS-CoV-2). The resulting disease is called emerging corona virus disease 2019 (COVID-19). In March 2020, the World Health Organization declared the corona virus (COVID-19) to be a global pandemic. In this research, a diagnostic kit was prepared that is used in the laboratory to detect infection with Acquired Corona Virus (COVID19) by the method of the enzyme immunoassay (ELISA). Conjugated secondary antibodies tagged with HRP which gives a color signal with the substrate added to it, its intensity depends on the amount of antibodies present in the pathological sample. We used a microtiter plate coated with the virus core and ns antigen and a conjugate product from Imbian Company, while the other of the kit components (reagents and buffers) were prepared in Al-Razi laboratories to be suitable for use. Tests were conducted on the prepared kit for 96 samples, including 55 samples for positive cases and 41 samples for negative cases, which were obtained from the specialized laboratories and patients. The tests showed conformity in the results compared to foreign kit used for this purpose and using the ELISA washer and reader devices available in Al-Razi center's laboratories. And by installing the method of preparation by fixing the method of preparation and obtaining identical results, Al-Razi center can produce pioneering batches and provide the laboratories of Ministry of Health of this type of diagnostic kits.

Cadmium is a toxic heavy metal, which is mainly absorbed by gastro intestinal tract (GIT). This absorption increases when there is a decrease in the level of iron in body stores. This study was carried to find out that the intake of ferrous sulphate affects the absorption of cadmium chloride from gastro intestinal tract (GIT) of mature rabbits exposed chronically to polluted water with cadmium chloride (CdCl2). Four to five months old rabbits were equally divided into three groups: Control (A), this group offered ordinary tab water, the second treated group (B), offered tap water containing 80 parts per billion (ppb) CdCl2. The third treated group (C), offered tap water containing (80 ppb) CdCl2 with 4mg of ferrous sulphate that are given orally once a day. Cadmium concentration in serum was measured using atomic absorption instrument at the end of experiment which extended for 8 weeks. The results revealed that Cd concentration was increased significantly in the 2nd group (B) as the CdCl2 level in serum reached 2.057 ppb, while it decreases significantly in the 3rd group (C) where the level decreased to 0.455 ppb compared to control group (A). These findings concluded that the supplementing food with iron compounds led to a decrease in serum cadmium levels, where ferrous competes with the absorption of Cd.

The current study aims to improve the quality of wastewater collected from the raw, primary sedimentation tank and secondary sedimentation tank of Hamdan wastewater treatment plant, Basrah, Iraq. Heavy metals contamination is one of the most critical environmental issues. Therefore, appropriate steps need to reduce pollutants in wastewater to acceptable levels before being discharged to the environment. Several treatment methods have been developed recently to adsorb these pollutants. This study reviews the ability of polymer, a new resin (3,3'-(1,4- phenylene) bis(1-(4-(4-hydroxybenzylidene) amino) phenyl) prop-2-en-1-one) to adsorb copper, cadmium and lead from wastewater. The new Schiff base was prepared on polyurethane foam diagnosed by FT-IR and 1H-NMR spectroscopic methods. The polymer has excellent sorption capacities due to the large specific surface area, chemical composition and the removal metals efficiency in varying proportions. For Cd2+, the efficiency removal was 100% in wastewater and the efficiency of removing Cu2+ was 100% in raw wastewater and primary sedimentation tank. The removal efficiency of Pb2+ was 86%, 64% and 79% 79% in raw sewage, primary and secondary sedimentation tank respectively.

Inflammatory bowel disease (IBD) is a chronic inflammatory disorder divided into two types, Ulcerative colitis (UC), and Crohnʹs disease (CD). In total, 80 blood samples were collected for this study, 40 blood samples were collected from IBD and 40 blood samples were collected from healthy individuals as. Genomic DNA extracted from whole blood and (FokI) polymorphism was detected by polymerase chain reaction Restriction fragment length polymorphism (PCR- RFLP). The results of the statistical analysis showed high frequency and percentage in age group (< 30 years) was 16 (40%) with significant (P≤0.05) when compeered with control group. The frequency and percentage of body mass index (BMI) for the patient group was (27.91 ± 0.45). While BMI was (27.31 ± 0.54) for the control group with highly significant association (p<0.01) between Iraqi patients with IBD when compeered with control group.(VDR- FokI) wild allele PCR products (without restriction site) have 265bp (F allele) and when digested and electrophoresis of the digestion products on agarose gel the resulted fragment size of 196bp, 265 bp and 69 bp (f allele).The genotype of FokI gene (TT/FF) was 14 (35%) (P-value=0.0039), TG / Ff 15(37.5%) (p= 0.0253), GG /ff 11(27.5%) (p=0.0039) in patient group which have a highly significant in frequency and percentage, as compared with control group.  In conclusion The FokI genes polymorphism was shown to be associated with an increased incidence of Iraqi patients with IBD when compeered with control group. The genetic factors of FokI gene polymorphism may have a role in inflammatory bowel disease.