This study was carried out at the Department of Ornamental Horticulture, Faculty of Agriculture, Cairo University during the two successive seasons of 2015 and 2016. The experiment was designed using a complete randomized blocks design. The objective of this study was to investigate the effect of NPK (recommended dose), biofertilizers (Nitrobien and Phosphorien) at 1,2 and 4 g/plant, compost and poultry manure at the rates of 5, 10 and 15 ton/ feddan (feddan=4200 m 2 ) on essential oil production and biochemical compositions of Cymbopogon citratus plants. The results showed that the average percentage of essential oil ranged between 0.140-0.300% at the first cut and 0.133-0.283% at the second cut of the two seasons, respectively. First cut (in August) resulted in more essential oil production than second cut (in October). Poultry manure produced the maximum essential oil yield/plant compared to control and the other fertilizer treatments. The main