Tuber yield of plants from three sizes of in vitro produced microtubers were compared to yields of plants from three sizes of greenhouse produced minitubers of Nicola and Russet Burbank. Microtubers were: small-size, 0.2-1.5 g; mid-size, 1.5-3.0 g; and large-size, >3.0 g. , and small-size minitubers gave the lowest tuber number, 409,375 tubers/ha −1. Regarding tuber yield, the type of tuber did not influence either cultivar. Nicola was influenced neither by type nor by size of tubers. Russet Burbank, however, was sensitive to size of tubers, especially, within minitubers. Large-size mini and microtubers were the most productive, 49 and 40 t ha −1
Our objective was to test whether the NPK levels in commonly used Murashige and Skoog (MS) media are optimal for microtuberiztion for the two cultivars tested. Two N (N 1 =841 and N 2 =1418 mg L −1) ; three P (P 1 =39, P 2 =76.9 and P 3 =115 mg L −1; and two K (K 1 =784 and K 2 =1518 mg L −1 ) media concentrations were evaluated for microtuberization. Higher levels of N 2 P 3 K 2 were more effective for microtuberization than the lower levels of N 1 P 1 K 1 in standard MS media. N 2 P 3 K 2 was the best concentration for all yield components studied, tuber number, tuber weight, and stem and root weight. For the two cultivars, our results suggest that higher NPK levels than t h o s e i n M S m e d i a w o u l d e n h a n c e i n v i t r o microtuberization, and that optimum NPK levels could vary for cultivars.Resumen Nuestro objetivo fue probar si los niveles de NPK en el medio comúnmente usado de Murashige y Skoog son óptimos para la microtuberización en las dos variedades probadas. Se evaluaron dos concentraciones de N en el medio (N1=841 y N2=1418 mg L −1 ); tres de P (P1=39, P2=76.9 y P3=115 mg L −1 ); y dos de K (K1=784 y K2=1518 mg L −1 ) para la microtuberización. Los niveles más altos de N2P3K3 fueron más efectivos que los más bajos N1P1K1 para tal fin en el medio estándar MS (Physiologia Plantarum 15:473-497, 1962). N2P3K2 fue la mejor concentración para todos los componentes de rendimiento estudiados, número y peso de tubérculo, y peso de tallo y raíz. Para las dos variedades, nuestros resultados sugieren que niveles más altos de NPK que los del medio MS estimularían la microtuberización in vitro, y que los niveles óptimos de NPK podrían variar para los cultivares. De las combinaciones de medios NPK probadas, N2P3K2 fue la superior de todas las evaluadas.
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