This experiment was performed on cucumber (Cucumis sativus L.) at Research Farm of the Faculty of Agriculture, Al-Azhar University (Assiut Branch), Assiut, Egypt (Longitude 031° 11' 09" E and latitude 27° 10' 39" N) in sandy clay loam soil. To evaluate nine F1 hybrids of cucumber by using molecular analysis. Cucumber hybrids were planted in March 2019 and 2020 for the summer seasons 2019 and 2020. The experiment was laid out in Randomized Complete Block Design (RCBD) with three replications. Randomly amplified polymorphic DNA was used to assess the genetic diversity and relationship among 9 cucumber hybrids. Five RAPD primers generated a total number of 34 bands, out of them 3 bands were polymorphic with 8.82% polymorphism. The total number of bands generated by each primer ranged from 5 to 9 bands with an average of 6.8 band per primer. Additionally, only two primers gave polymorphism among cucumber samples i.e., OPA-03 and OPA-08. Some genetic diversity measures were estimated based on RAPD molecular data. In this regard, the polymorphism information content (PIC) ranged for 0.05 (OPA-03) to 0.16 (OPA-08) with an average of 0.04 for all primers. The primer resolving power was 0.67 for OPA-03 and 1.11 for OPA-08, with an overall value of 1.78 for RAPD primers. In addition, diversity index was almost the same as PIC, with 0.04 (OPA-03) and 0.16 (OPA-08) and an average of 0.04 for all RAPD primers. .
Background: Antenatal surveillance using Doppler ultrasound velocimetry of the uteroplacental umbilical and fetus arteries has been commonplace. The cerebroplacental ratio has been investigated as a way to predict newborn outcomes. Aim and objectives: In this research, we wanted to see whether the cerebro-placental ratio may help predict perinatal outcomes in high-risk gestation with intrauterine growth restriction. Subjects and methods: This was case-control research done at Al-Hussein University Hospital's Department of Obstetrics and Gynecology; The obstetric color Doppler investigation might be examined in two groups of 100 high-risk pregnant wome: Group Ⅰ - study group: 50 high risk pregnant women with IUGR and Group Ⅱ - control group: 50 high risk pregnant women without IUGR. Results: CPR achieved significance at cutoff point of 1.03 for predicting IUGR with sensitivity of 87.2% and specificity of 71.6% with PPV 83% and NPV 79%. Conclusion: Intrauterine growth restriction in high-risk gestations, the cerebral-placental ratio has a significant prognostic value for perinatal outcome.
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