Image processing becomes one of the most important demands, requisites and exigencies in the 20th century, because of its usages in many and different parts of habitats and customs, so the enhanced image represents the main issue for scientists and researchers, where little methods, devices and programs were existing. According to this, our study was significantly concerned with the image processing; namely, the living cell imaged using different lenses on the confocal microscopy. The microscope was designed very well to give perfect resolution imaged taken from different situation (angles) and different lenses with the magnification (X = 40, 100 and 160) and various light sources (white light, laser red, laser blue, and laser green). From the results conducted and achieved it's clear that the lens with magnification 100 and near wavelength light color gives more information about the living cell (object). The histograms confirm vigorously these results, so this could solve and enhanced the resolution image in multi disciplines, scopes and avenues.