The objectives of this research were to determine the chemical composition of the extract of Macrotyloma uniflorum seeds by using Gas chromatography–Mass Spectrometry analysis (GC-MS). GC-MS is a matchless method to study and measure the quantity of organic volatile and semi-volatile compounds. Gas chromatography is employed to separates mixtures into individual components employing a temperature-controlled capillary column. Mass spectrometry is utilized to recognize a variety of components from their mass spectra. In the present study, volatile/semivolatile compounds present inM. uniflorum seed extract were analyzed. M. uniflorum seed extract isextracted by the soxhlet extraction method and then analyzed by GC-MS. A total of 28 compoundswere found and quantified in this study. The major bioactive compounds in M. uniflorum seed extract are: 1,7-Octadiyne, 1-Propanone, 1-phenyl-3-[2-( phenylmethoxy)phenyl] -(16.93%), Benzene, 1-methyl-4-(1,2,2-trimethylcyclopentyl)-, (R)-, Benzene, 1-(1,5-dimethyl-4-hexenyl)- 4-methyl- (13.88%) and à-Farnesene, 1,3,6,10-Dodecatetraene, 3,7,11-trimethyl- (Z,E)- (12.68%). The present investigation revealed that M. uniflorum was composed of variety of metabolites and therapeutic active substances as well as novel substances. These substances could be isolated and further empirically evaluated to confirm their biologic and medicinal activities as well as investigate their mechanism of action.

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