BACKGROUND: Solid fat was produced from mustard oil and palm stearin through lipase-catalyzed reaction, in which linoleic acid was intentionally incorporated. For optimizing the reaction condition of melting point and ω6/ω3 fatty acids, response surface methodology (RSM) was employed with three reaction variables such as substrate mole ratio of mustard oil (MO) to palm stearin (PS) (X 1 ), reaction temperature (X 2 ) and reaction time (X 3 ).
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