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This paper describes a simple, precise, and validated high-performance liquid chromatographic method for the simultaneous quantitative determination of amcinonide and benzyl alcohol in pharmaceutical preparations. The separation was achieved using a Zorbax reversed-phase column (5 m, 250x4.6mm) at room temperature with an isocratic mixture of deionized water and acetonitrile (70:30 v/v) at a flow rate of 1.5ml/min and detection at 254 nm. The limits of quantification for amcinonide and benzyl alcohol were found to be 4-30 g/ml and 80-600 g/ml, respectively. The validity of the method was evaluated in terms of linear regression analysis, precision, specificity, accuracy and ruggedness. The developed methodology is an economic, time-saving, straight forward and precise assay for the determination of amcinonide and benzyl alcohol in pharmaceutical preparations. It can be readily utilized for quality assurance and R&D laboratories of pharmaceutical and cosmetic industry.

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5-Fluorocytosine (5-FC) is used for the treatment of several infections. It is extremely important to monitor blood level concentration for maximum activity to avoid its side effects. A simple, faster, and more accurate analytical method is developed and validated using high-performance liquid chromatography with UV detection in a very low-volume serum sample. Exactly 50 µL of serum was precipitated with 5% trichloroacetic acid. After mixing and centrifugation, 20 µL of supernatant was injected into the HPLC column. Detection was performed at 280 nm. The method is very specific and free from interfering substances due to different drugs and their different circulating metabolites. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.50 μg/L and 1.0 μg/L, respectively. The method was linear in the range of 5 -150 μg/L in the serum sample. In method comparison, the correlation coefficient r 2 was 0.999 and the percentage recovery was 90% -105% on four levels of the quality control samples. Within run and between run precision was found to be less than 2.2% at four different concentrations (5, 25, 50, and 100 μg/L). A simple, faster, and more accurate HPLC-UV method is developed which is very useful for monitoring 5-FC concentration in low volume serum samples without evaporation step and ion exchange chromatography within minutes.