This study aims to analyze the hormone profile of FSH, LH and E2 to the eel after exogenous hormone stimulation and blood glucose levels. Six formulated treatment applied with combination of dopamine antagonize 10 mg.mL–1 (A), estradiol (E2) 3 mg.mL–1 + A (EA), metyltestosteron (MT) 3 mg.mL–1 + A (MTA), hCG 2 mg.mL–1 + EA (hEA) and hCG 2 mg.mL–1 + MTA (hMTA), with (F) physiologis 0.9% NaCl to control. Six group eel (200±15g) reared in a concrete tank with a capacity of 3,400 liters and filled with sea water of 35 mg L–1 as much as 2000 liters. Eels injected 1 mL.kg–1 hormone by intramusculary, were feed to apparent satiation daily for 10 weeks. The study used Completely Randomized Design with one treatment factor, namely hormonal factor and its combination. Fish blood that ware directly concentration to FSH, LH and E2, the enhanced significantly high in the blood plasma on treatment hMTA and hEA P<0.05. Glucose concentration in the blood palsma is high enough in a row on a formula hMTA 67.33 mg.dL–1 and significantly different to P<0.05. The result indicates that induction of exogenous hormone (hMTA) improve FSH, LH and E2. FSH and LH profiles show permutation patterns during the development of eel gonad, beginning with the increase of FSH in the early phases of gonadal development. LH profile moves in line with the increase in E2 during gonadal maturation process eels (A. bicolor bicolor), with blood glucose levels in the normal range. AbstrakThis study aims to analyze the hormone profile of FSH, LH and E2 to the eel after exogenous hormone stimulation and blood glucose levels. Six formulated treatment applied with combination of dopamine antagonize 10 mg.mL–1 (A), estradiol (E2) 3 mg.mL–1 + A (EA), metyltestosteron (MT) 3 mg.mL–1 + A (MTA), hCG 2 mg.mL–1 + EA (hEA) and hCG 2 mg.mL–1 + MTA (hMTA), with (F) physiologis 0.9% NaCl to control. Six group eel (200±15g) reared in a concrete tank with a capacity of 3,400 liters and filled with sea water of 35 mg L–1 as much as 2000 liters. Eels injected 1 mL.kg–1 hormone by intramusculary, were feed to apparent satiation daily for 10 weeks. The study used Completely Randomized Design with one treatment factor, namely hormonal factor and its combination. Fish blood that ware directly concentration to FSH, LH and E2, the enhanced significantly high in the blood plasma on treatment hMTA and hEA P<0.05. Glucose concentration in the blood palsma is high enough in a row on a formula hMTA 67.33 mg.dL–1 and significantly different to P<0.05. The result indicates that induction of exogenous hormone (hMTA) improve FSH, LH and E2. FSH and LH profiles show permutation patterns during the development of eel gonad, beginning with the increase of FSH in the early phases of gonadal development. LH profile moves in line with the increase in E2 during gonadal maturation process eels (A. bicolor bicolor), with blood glucose levels in the normal range.

<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p class="NoParagraphStyle">The aim of the study was to stimulate eel gonad growth by intramuscularly injection with a hormonal combination of human chorionic gonadotropin (hCG), methyltestosterone (T), estradiol (E₂) and antidopamine (A) meanwhile 0.9% NaCl solution was used as control. Estradiol concentration in the blood serum were measured by enzyme-linked immunosorbent assay (ELISA). Stimulation with hCG 20 IU/kg in combination with MT 3 mg/kg and 10 μg/kg antidopamine (hTA treatment) very effective for increased the growth of gonads with indicators gonadosomatic index (GSI) of 4.80%, hepatosomatic index 1.01% and concentration of E₂ 2.49±0.67 ng/mL were significantly different (P&lt;0.05) than others treatment after ten weeks of application.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Key words: hormone, gonad growth, maturation, Indonesian short finned eel</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Penelitian ini bertujuan untuk mengetahui pertumbuhan gonad yang distimulasi oleh kombinasi <em>human chorionic gonadotropin</em> (hCG), metiltestosteron (MT), estradiol (E₂) dan antidopamin (A). Ikan sidat disuntik dengan hormon dan 0,9% NaCl sebagai kontrol secara intramuskular. Pengukuran konsentrasi hormon E₂ dalam serum darah dengan <em>enzyme-linked immunosorbent assay </em>(ELISA). Stimulasi dengan hCG 20 IU/kg dikombinasi dengan MT 3 mg/kg dan antidopamin 10 μg/kg (perlakuan hTA) sangat efektif untuk feminisasi dan meningkatkan pertumbuhan gonad dengan indikator indeks gonadosomatik (GSI) 4,80%, indeks hepatosomatik 1,01% dan konsentrasi E₂2,49±0,67 ng/mL, secara signifikan berbeda pada taraf P&lt;0,05 dibandingkan perlakuan lain setelah sepuluh minggu aplikasi.</p><p class="NoParagraphStyle"> </p><p>Kata kunci: hormon, pertumbuhan gonad, maturasi, sidat</p>

Penelitian ini bertujuan menganalisis fase spermatogenesis sidat hasil induksi kombinasi hormon 17α-metil testosteron (MT) dan anti dopamin (AD). Tiga perlakuan diatur sebagai berikut: AD 10 µg.kg–1 (A); AM 3 mg.L–1 (AM) dan larutan fisiologis NaCl 9 mg.L–1 sebagai kontrol (F). Sidat (200±15 g) dikelompokkan menjadi tiga unit percobaan yang dipelihara dalam bak beton berkapasitas 3.400 liter berisi air sebanyak 2000 liter dengan salinitas 35 ppt. Dosis hormon yang digunakan 1 mL.kg–1, diinjeksikan secara intramuskular pada pangkal sirip punggung. Sidat diberi pakan pellet dengan kadar protein 46% secara at satiation sekali sehari selama 10 minggu. Gambaran histoanatomi gonad sidat perlakuan AM manifestasi dari tahapan spermatogenesis, spermiogenesis dan spermiasi hingga minggu ke-10. Ditemukan gonad matang yang mengandung spermatozoa, dengan nilai IGS 1,40% setelah sepuluh minggu perlakuan. Pemanfaatan kombinasi hormon metiltestosteron dan anti dopamin efektiv merangsang proses spermatogenesis hingga fase spermiasi.

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