The current study was conducted at Wasit province, Iraq during the period from 28 October 2020 to 28 December 2020. The study included one hundred stool samples taken from questionable patients of Entamoeba histolytica and Helicobacter pylori. Samples were collected from patient attended to General Hospital of Martyr Fairuz at Hay district , Saeed Health center and Hospital of Al-Kut. All samples have been checked using direct smear lugol's iodine stain for Entamoeba histolytica and dipstick for Helicobacter pylori. The result showed that 40 samples (40%) out of 100 patients were gave positive by rapid test cassette; females 23 (23%) positive samples and males 17(17%). While 14 samples (14%)out of 100 samples were positive for Entamoeba histolytica. The highest infection in females 8(8%) and the lowest in males 6(6%). The result showed 10 samples (10%) out of 100 patients positive for both E. histolytica and H. pylori. The highest infection was recorded in females 6(6%) and the lowest in males 4(4%).The highest infection of H.pylori in age group (39 and more) years ; females recorded 6(6%) and males recorded 6(6%), while the lowest infection in group (>1) years old ; females recorded 1(1%) and males recorded 1(1%). The infection rate of Entamoeba histolytica was highest in age group (1-13) years old ; females was recorded 1(1%) and males was recorded 4(4%) , while the lowest in age group (26-39) years old ; females recorded 1(1%) while males without infection. Infection with both E.histolytica and H.pylori was recorded the highest in group (39 and more) years old ; females was recorded 3(3%) and males was recorded 2(2%) while the group (>1) years old do not recorded infections.

Trichomoniasis is a common sexually transmitted disease (STD) caused by Trichomonas vaginalis. The current study was conducted during the period from January 2022 to July 2022 to investigate the infection of Trichomonas vaginalis  in Iraqi women at Wasit province . A total of 100 women of different ages were enrolled in this study. The vaginal swabs were collected from each woman. A consent form which included age, area of residence, educational level , in addition to the symptoms were obtained from the participants. By Wet mount examination , 4% infection rate with Trichomonas vaginalis. The age class of 20-30 years had the highest rate of infection (3%); while infection among patients from urban resident (3%) was higher than of rural resident (1%). Women with primary educational level showed higher prevalence of Trichomonas vaginalis (2%) than other levels and status. About 86 % of the studied women had vaginal discharge , while 81% of them had vulvular itching . Dysuria was Other less frequent symptom. These results indicated the low prevalence of Trichomonas vaginalis among Iraqi women at Wasit province

This study was concerned the describe of an current PCR method for specific detection of Tichomonas vaginalis DNA into a rapid nested PCR assay, and to estimate the recent prevalence of T. vaginalis according to studying different factors that effect on vaginal trichomoniasis in women complaining of vaginal discharge. Vaginal swab samples were collected from 100 patients lived at Wasit province, during the period from January, 2022 to July, 2022. Demographic data, clinical and socioeconomic status were collected from the patients using a structured questionnaire. Total genomic DNA was isolated from 100 samples of vaginal swab randomly chosen to specific detection of T. vaginalis DNA into a rapid nested PCR. All samples were examined by the direct microscopic examination (wet mount). The vaginal swabs examined by wet mount and nested PCR to detect the presence of T. vaginalis. Of the 100 women, 29 were positive by nested PCR giving the prevalence rate of (29%) with and the direct microscopic examination (wet mount) showed 4/100 (4 %) positive case. Their ages were different. Women aged (20-30)years had significantly higher prevalence of trichomoniasis (14%) than other age groups.

The present study aimed to investigate the presence and prevalence of Cryptosporidium among immunocompromised patients in Wasit Hospitals and health centers to determine the best method for its diagnosis. The study started from the 1st of August to the end of November 2020. One hundred forty stool samples were collected from immunocompromised patients from both genders who attended the hospital and health centers at Wasit province. Stool samples were inspected by modified acid-fast stain as a standard method, enzyme linked immunosorbent assay (ELISA) and Molecular Nested PCR method. Modified Ziehl-Neelsen technique revealed 46 (33%) positive cases for Cryptosporidium oocysts, of 85 males and 55 females, highly significant relationship was found between the genders and between different age groups of infection with a highly significant difference between rural and urban area. The prevalence of Cryptosporidium was 57.14% using nested PCR and 38.46% for ELISA in comparison with MZN method. It is concluded that cryptosporidiosis found to be endemic in Wasit Province for the first time and the nested PCR was the most reliable technique for its diagnosis.

The present study aimed to identify the relationship between Cryptosporidium parvum parasite and Helicobacter pylori in patients at Wasit province and its environs. One hundred stool samples were collected (male and female) from suspected diarrheal patients of parasitic and bacterial infection during the period October 2020 to April 2021 who attended to Al-Karamah Teaching Hospital at Wasit province and General Hospital of Martyr Fairuz at Hay district.The investigation of the oocysts of the Cryptosporidium parvum parasite was carried out by staining with Modified Ziehl Neelson method and also detecting of Helicobacter pylori by using dipstick. The results of our study revealed that 55( 55%) was positive for C. parvum and 48 (48%) was positive for Helicobacter pylori and the co-infections between the parasite and bacteria reached to 32( 32%). The age group(1-20 years) showed the highest11 (11%) prevalence rate while the lowest prevalence was in patient with age group (21-40 years) reached to 6(6%) interplay infections between the parasite and bacteria .