Copper oxide nanoparticles (CuONPs) have a wide range of uses in agricultural applications. Nanocurcumin (NCur) acts as an antioxidant treatment. The goal of the study is to reduce the toxicity resulting from the use of CuONPs as nanopesticides on living organisms by inducing changes in the morphological shape of CuONPs or treating it with NCur. So, we induced a comparative study between three shapes of CuONPs: CuO nanosphere (CuONSp), CuO nanosheet (CuONS), and CuO nanoflower (CuONF). We characterize each nano-form by using X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (HRTEM), and Zetasizer HT device; 36 rats were divided into six groups (n = 6): 1st group was the control group; 2nd group received 50 mg/kg/day of NCur orally for 30 days; 3rd, 4th, and 5th groups received orally 50 mg/kg/day of CuONSp, CuONS, and CuONF, respectively, for 30 days; 6th group received 50 mg/kg/day CuONSp plus 50 mg/kg/day of NCur orally for 30 days. An elevation occurred in malondialdehyde (MDA), liver and kidney functions, tumor necrosis factor-alpha (TNF-α), and B-cell lymphoma 2 (Bcl2) by CuONSp > CuONS > CuONF, respectively. An inhibition occurred in glutathione (GSH), superoxidase (SOD) catalase (CAT), apoptotic Bax gene (Bax), histopathological, and ultrastructural alterations by CuONSp < CuONS < CuONF, respectively. NCur ameliorated these alternations. In conclusion, CuONF is a better form compared to other forms of nanopesticide in agriculture due to its lower toxicity. NCur decreased the biological alternations which induced by CuONSp due to its antioxidant and anti-apoptotic properties. Graphical Abstract

Copper oxide Nanoparticles (CuONPs) are used in different agricultural applications. Large amounts of CuONPs cause organ dysfunction in animals. Our study aim to compare between the toxic effects of CuONanSphere (CuONSp) and CuONanoFlower (CuONF) as new nano-pesticides, determine a less toxic form when used in agricultural applications. To characterize CuONSp and CuONF, we used X-ray diffraction (XRD), Field emission scanning electron microscopy (SEM), and High resolution transmission electron microscopy (HRTEM) and Zeta-sizer device.18 adult male albino rats were divided into three groups (n = 6), (I) control group, (II) and (III) groups were given orally 50 mg/kg/day of CuONSp and CuONF 30 days respectively. CuONSp induced oxidant-antioxidant abnormalities, including an increase in malondialdhyde (MDA) and a decrease in glutathione (GSH) in comparison to CuONF-treated one. CuONSp induced an increase in liver enzymes activities compared to CuONF. Tumour necrosis factor-alfa (TNF-α) detected an increased in liver and lung compared to CuONF. However, histological examinations revealed changes in CuONSp group than CuONF group. Changes in immune-expressions of TNF-α, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kβ) and tumour suppressor gene (p53) were also more identified in CuONSp group than CuONF group. Ultrastructural studies of liver and lung tissues marked alternations were observed in CuONSp group than CuONF group. In conclusion, CuONSp induced biological alternation in liver and lung more than CuONF. So, CuONF is less toxic compared to CuONSp when used as nano-pesticide in agricultural applications. Graphical abstract

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