Present investigation was carried out to examine the efficacy of different bio-agents on vegetative and flowering parameters of chrysanthemum at Department of Floriculture and Landscaping, College of Horticulture and Forestry, Jhalawar. For this formulations of different bio-agents like Pseudomonas fluorescens (125g and 175g and 250g/plot area), Trichoderma harzianum, Trichoderma viride, Trichoderma longibrachiatum, Bacillus subtilis used as root application. Pseudomonas fluorescens (250g) treatment was found effective and significantly affected growth and flowering parameters. Plant treated with 250g Pseudomonas fluorescens had maximum plant height (35.27 cm), number of leaves per plant (161.20), leaf length (9.80 cm), leaf width (7.44 cm), main stem diameter (1.22 cm), number of primary branches per plant (7.83) plant fresh weight (281.65 g), plant dry weight (87.67 g). While, flower diameter (7.34 cm), fresh weight of flower (31.56 g), dry weight of flower (4.06 g), In-situ life of flower (19.80 days) were recorded in Trichoderma viride 250g/plot area.