The effect of dietary supplementation of Cu and Zn on semen quality parameters and certain bio-chemicals parameters were evaluated in Murrah bulls. Twelve mature Murrah bulls (4-6 years of age) were divided into three groups (n=4) T1, T2, and T3 based on semen volume and concentration and were fed as per ICAR standard (2013). However, the animals were supplemented with 0%, 25%, and 50% Cu and Zn above the basal diet in T1, T2 and T3 groups, respectively for 180 days. Quantitative and qualitative characteristics of semen, blood and seminal plasma antioxidant status, blood and semen minerals (Cu, Zn, Ca and Mn) were determined in experimental Murrah bulls. Semen ejaculate volume (mL) increased in T2 and T3 while sperm concentration (million/mL), intact acrosome (%), HOST reacted spermatozoa (%) increased in T3 group compared to T1 and T2 groups. No difference was observed in mass motility, pH, live spermatozoa (%) in semen sexual behaviour except dismounting time which was decreased in T3 than T1 and T2 (plessthan0.05). SOD activity in blood plasma and LPO activity decreased in seminal plasma in both T2 and T3 groups than T1, whereas catalase activity did not show any significant difference. Cu and Zn supplementation in T2 group improved plessthan0.05) Zn level in blood and seminal plasma and Cu level in blood plasma only, but not in seminal plasma. Therefore, it can be concluded that supplementation of Zn and Cu at 50% above the recommended levels of ICAR (2013) improved the qualitative and quantitative attributes of semen in Murrah bulls.