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The data article contains the experimental data and images on the callus cell, shoot and stem proliferation from pineapple crown slice and banana inflorescence in vitro. Investigated data are related to the research article “Effects of benzylaminopurine and naphthalene acetic acid on proliferation and shoot growth of pineapple (Ananas comosus L. Merr) in vitro” Alsaif et al. (2011) [1] and “Plantlet Production through Development of Competent Multiple Meristem Cultures from Male Inflorescence of Banana, Musa acuminta cv. ׳Pisang Mas׳(AA)” Wirakarnain et al. (2008) [2]. In the experimental data 1, physiological, (shoot weight, number length and stem proliferation) biochemical (total sugar and chlorophyll) and nutritional ((K+ and NO3−) data using BAP, MS medium and NAA growth regulators in pineapple have been explored. In the experimental data 2, physiological, (callus weight, shoot number and length) biochemical (total sugar, chlorophyll, total phenol, DPPH) and nutritional (K+ and NO3−) data employing BAP +IAA, MS medium and NAA growth regulators in banana have been exhibited. Overall quantitative measurement was observed by Spectrophotometer. In the experimental data, BAP was shown the best effective hormone for the both pineapple and banana explants regeneration.

In this study, we investigated the effects of 2,4-Dichlorophenoxy acetic acid (2,4-D) treatment on selected physiological parameters and fruit quality of wax apple fruits under field conditions. Foliar spray of 2,4-D at 0, 5, 10 and 20 mg L -1 concentrations were used from bud stage to the 3rd week of fruit development. Physiological parameters determined included net photosynthesis, stomatal conductivity, chlorophyll content and chlorophyll fluorescence. Net photosynthetic rate and photosynthetic efficiency via chlorophyll fluorescence significantly (p≤0.05) increased with 5 and 10 mg L -1 2, 4-D treatments. Furthermore, 5 mg L -1 2,4-D reduced bud and fruit drop, increased fruit weight and dry matter content in leaves. An improved fruit set, enhanced fruit growth, faster color development and advanced maturity were also recorded in treated plants. In addition, fruit yield increased by 39 and 48% with 5 and 10 mg L -1 2,4-D treatments, respectively. Fruit K + content, total sugar, protein content and chalcone synthase (CHS) activities increased with 2,4-D treatments. Increased leaf soluble protein and elevated sucrose phosphate synthase (SPS) activity were also observed in 5 mg L -1 treated plants. Positive correlation between photosynthesis rate and SPS activity of leaves (R 2 =0.98) and between CHS activity and color development of fruits (R 2 =0.98) were recorded in 5 mg L -1 treatments. The results also suggested that 5 and 10 mg L -1 2,4-D treatments are promising for enhancing plant productivity and fruit quality in wax apple trees under field conditions. ABSTRAK Dalam penyelidikan ini, kami mengkaji kesan aplikasi 2,4-Dichlorophenoxy asid asetik (2,4-D) pada parameter fisiologi yang dipilih dan kualiti buah jambu madu di bawah keadaan ladang. Semburan foliar 2,4-D dengan kepekatan 0, 5, 10 dan 20 mg L-1 digunakan pada peringkat putik hingga ke minggu ke-3 pembentukan buah. Parameter fisiologi yang ditentukan termasuk fotosintesis bersih, kekonduksian stomata, kandungan klorofil dan klorofil pendarfluor. Kadar fotosintesis bersih dan kecekapan fotosintesis melalui klorofil pendarfluor meningkat dengan ketara (p≤0.05) dengan rawatan 5 dan 10 mg L-1 2,4-D. Tambahan pula, rawatan 5 mg L-1 2,4-D mengurangkan keluruhan putik dan buah dan meningkatkan berat buah dan kandungan bahan kering dalam daun. Set buah yang ditambah baik, pertumbuhan yang dipertingkatkan, pembangunan warna yang lebih cepat dan kematangan yang maju juga dicatatkan bagi tumbuhan yang dirawat. Di samping itu, penghasilan buah telah meningkat sebanyak 39 dan 48% dengan rawatan masing-masing 5 dan 10 mg L-1 2,4-D. Kandungan K + , jumlah gula, kandungan protein dan aktiviti kalkon sintase (CHS) dalam buah meningkat dengan rawatan 2,4-D. Peningkatan protein larut daun dan aktiviti sukrosa fosfat sintase (SPS) juga diperhatikan dalam 5 mg L-1 tumbuhan yang dirawat. Hubungan positif antara kadar fotosintesis dan aktiviti SPS daun (R2=0.96) serta antara aktiviti CHS dan pembangunan warna buah (R2=0.98) dicatatkan dalam rawatan 5 mg L-1. Keputusan ...

The study was conducted to evaluate the comparison of the callus cell, shoot and stem proliferation from pineapple crown slice and banana inflorescence in vitro using NAA and BAP at different concentration. The pineapple shoot number and weight was higher in BAP at 2.0 mg/l than control and NAA 0.2 mg/l. However, the BAP + IAA (10mg/l + 1 mg/l) concentration produced higher callus cell weight, shoot number and stem length than MS medium (without hormone) and NAA 1mg/l concentrations in banana. Moreover, it has been shown that the sugar and chlorophyll content were higher in BAP treated hormone than MS medium and NAA 0.2mg/l concentration in pineapple. In addition to that the nutrient content (K+ and NO3-) was exhibited higher in the 2 mg/l BAP treated explants than MS (control) and 0.2 mg/l NAA treated explants in pineapple. Callus tissue weight was maximal in BAP (10mg/l) + IAA (1mg.l) treated explants compared to the NAA (1 mg/l) and MS treated explants in banana. Besides, shoot number and stem length were higher in the BAP (10mg/l) + IAA (1mg/l) treated explants than NAA (1 mg/l) and MS (control) media in banana. The highest sugar and chlorophyll content was recorded in BAP (10mg/l) + IAA (1mg/l) treated explants in banana. Meanwhile, the total phenol content was significantly increased by BAP treatment. K + and NO3-content in explants were significantly increased by BAP and NAA treatments in banana. The result showed that the DPPH radical scavenging activity increased with hormone application. Finally it seemed that BAP was the best effective hormone for the both pineapple and banana explants regeneration. Therefore the highest shoot and stem was regenerated in BAP (2mg/l) in pineapple.