This study was carried out to investigate the immune status of heart disease patients infected with toxoplasmosis. One hundred fifty samples of both heart disease patients and controls which had been tested by ELISA technique to detect anti-Toxoplasma Abs (IgG and IgM). The positive and negative toxoplasmosis samples were tested to detect the level of IL-12, IL-23 and TGF β-3. Results showed there one samples clarified that seropositive for IgM antibodies while 64 (64%) heart disease patients were seropositive for IgG antibodies and for toxoplasmosis only patients were 20 (40%) and 30 healthy as a (controls) were seronegative for IgG antibodies with significant differences (P< 0.05). Serum level of IL-12 was recorded an increase in a group of heart disease patient with toxoplasmosis (275.4 ±42.3 pg/ml) with highly differences of significant (P < 0.05) also IL-23 level was increased in a group of heart disease patient only (80.3 ±42.3 pg/ml) with no significant differences .TGF β-3 levels was highly in heart disease patient with toxoplasmosis (381.8 ±44.0 pg/ml) with significant differences at (P< 0.05).