Many soils have the problem of high phosphate retention values (> 85%) thus P is not available for plants. The activity of phosphate solubilizing microbes (PSM) is able to facilitate the availability of phosphorus in the soil. The ability of each microbe to dissolve inorganic phosphate varies and depends on the type of microbe and the microbial growth factors. In nature, almost all forms of life are surrounded by various types of sounds and they also interact with these sounds. Previous research found that the soil that had been applied with the murottal Al-Qur’an sound for 2 hours per day for 7 days increased the total population of soil microbes 23.08% more than without applying murottal sound. This research was carried out in the laboratory of the Faculty of Agriculture, University of Muhammadiyah Sumatera Utara from April to August 2019. This treatment was sounds on 9 rhizosphere of corn, were put into the box. The sounds were treat every morning during 2 hours at 08.00 -10.00 o’clock and compared with 9 rhizosphere of corn was not treated with sounds. The result showed that sound treatment affected the growth of phosphate solubilizing microbes and all microbes that were not pathogenic to plans.

Metabolic protein is the main source of truly absorbed amino acids. The determination of metabolic protein and metabolic acids is an important, but very difficult task. To do this, you need to know the exact number of incoming protein fractions in the small intestine as part of the duodenal fl ow of chymus, their digestibility, the fl ow of metabolism of essential amino acids from the intestine to the bloodstream and the coefficients of their use for milk protein biosynthesis and maintenance of life. The purpose of the research was to determine the need of lactating cows for metabolic essential amino acids by factorial method. During the researches, developed standards needs to metabolic essential amino acids for lactating cows (g/kg) milk with a content of 3,3 % (33 g/kg) crude protein and 3,15 % (31,5 g/kg) of pure protein and to maintain 1 kg/body weight 0,75, which allows to calculate the daily requirement in the absolute number of amino acids (g). The assessment of security of rations of lactating cows metabolic lysine and methionine has been shown coincidence with the norms of needs in the amount of 93 %; daily milk yield of natural milk under this provision of lysine and methionine were 38,7 % and 39,4. The availability of metabolic methionine was at the level of 77-85 %, which is explained by the unprocessed coefficients of transformation of metabolic methionine into pure methionine of milk protein. In the total duodenal fl ow of amino acids approximately 60-70 % is microbial protein and 30-40 % is non-degradable protein in the rumen. It has been found that the digestibility of dry and organic matter in the rumen and the general digestive tract in relation to UDP:DP 35: 65 % crude protein is 2-3 % (absolute) higher than for UDP:DP = 50:50 % crude protein.

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