This study sought to ascertain factors that affect facilitation of agricultural programmes as perceived by different gender groups among facilitators of FADAMA 111 development programme in Enugu State. It ascertained the perception of the gender groups on the organizational, job related and employee related factors. The data were analyzed with mean, standard deviation and t-test. Results indicate that out of the various identified organizational factors that affect facilitators in Enugu National Fadama Development Project III only one, monitoring and Evaluation System (t=-3.08*) was perceived differently by both men and women facilitators. From the various identified job related factors that affect facilitators in Enugu NFDP III only insufficient authority (t= 1.17) was perceived differently by both men and women facilitators. The results also show that from the various identified employee/personnel factors that affect facilitators in Enugu NFDP III, involvement in social meetings, involvement in house chores, size of family, relationship with spouse, proximity to client and extended family burden were perceived differently by both men and women facilitators. Based on the findings of the study, some factors were identified to affect the facilitators differently because of their genders differences. Hence, there is need to consider these factors and ensure balanced working conditions for both men and women facilitators in the NFDP III. This will help to ultimately improve their job performance.