Wastewater is a major environmental problem. Swine slaughterhouses generate a large volume of wastewater with high an organic load and nutrients. It therefore has the potential to cause environmental problems. The effects of swine slaughterhouse wastewater conditions on algae growth are evaluated. Microalgae, Chlorella vulgaris TIST8580 in water mixtures Tris acetate phosphate medium (control) and in diluted sewage from a slaughter house with the ratio of 25:75 and 50:50 (sewage: water) were experimentally studied. We used One-way ANalysis Of Variance (One-way ANOVA) and Two-ways analysis of variance (Two-way ANOVA) techniques for testing the differences between the 3 cases of the culture conditions used. It was found that from the One-Way ANOVA, the average number of cells of algae in wastewater in the 25% and 50% groups is not significantly different but both of these groups are significantly different from that of the control group. In addition, from the Two-Way ANOVA, we found that unlike the control group, the same kind of data in both 25% and 50% groups had no significant difference. This implies that the characteristic growth of algae in these two group do not significantly change over the culture period. We believe that our finding could benefit the researchers to properly design experiments especially for the case of resource limitation.