Our results suggested that both epigenetic modifications and Helios gene expression may have important roles in the pathogenesis of RA through their effects on Foxp3 gene expression.

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Objective: To investigate and compare the prognostic value of P53 and Ki67 markers with age and metastasis and survival in patients with breast cancer in Kermanshah, western Iran. Methods: In our study on 116 patients with breast cancer that all of them were women and kind of pathology was invasive ductal carcinoma and patients had Her2 positive. The expression of ki67 marker and p53 genes were determined by immunohistochemistry. Statistical analysis was performed with SPSS version IBM 19 and Disease free survival was calculated using the Kaplan-Meier method and log-rank test. Patients were followed up to 5 years. Results: The age mean of patients was 46.5±10.75.Of 116 patients, 23 patients (19.8%) had breast cancer with metastasis and 93(80.2%) without metastasis. expression of P53 (50%) in 58 patients was positive and 58(50%) was negative. there is a statistically significant relationship between the p53 and metastasis (P<0.05), but no for Ki67. The over expression of p53 protein and Ki67 had no significant relationship with survival rate (P>0.05). Conclusions: The results showed that the Ki67 marker and P53 protein are important factors in the breast cancer patients with emphasis on therapeutic agents.

Introduction: Stroke is one of the most common causes of death worldwide. Inflammation and apoptosis play an important role in the cascade of ischemic stroke. Aim: The aim of the present study was to evaluate the pretreatment effects of Lamium album (L. album) extract on caspase-3 and cyclooxygenase-2 (COX-2) expression, infarct volume, and neurological deficit score in a rat model of middle cerebral artery occlusion (MCAO). Materials and methods: Wistar male rats were randomly divided into three groups: 1) MCAO group (1 h after MCAO, reperfusion was allowed for 24 h by retracting the thread); 2) L. album + MCAO group [receiving L. album extract (100 mg/kg via intraperitoneal) for a week before MCAO]; 3) sham group. The expression level of caspase-3 and COX-2 in the core, penumbra, and subcortex regions was measured by real time-PCR technique. Infarct volume and neurological deficit score were also assessed. Results: The mRNA expression of caspase-3 in the core, penumbra, and subcortex regions in L. album group was significantly reduced compared to MCAO group (p&lt;0.05). Expression level of COX-2 in the subcortex of the rats exposed to L. album was statistically decreased relative to MCAO group (p&lt;0.05). Infarct volume in the core, penumbra, and subcortex was significantly reduced in the L. album group compared with MCAO group (p&lt;0.001, p&lt;0.001, p&lt;0.05, respectively). Neurological deficit score was remarkably decreased in the L. album group in comparison with the MCAO group (p&lt;0.05). Conclusions: It appears that pretreatment with L. album extract may attenuate brain tissue damage after ischemic stroke. The potential protective effects of this plant extract against this condition might be in part attributed to its anti-inflammatory and anti-apoptotic activities.