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The mycoflora and mycotoxins contamination of commercial maize and rice grains collected from local markets of the major five zones of the province of Cairo, Egypt, represented by 20 different districts were studied. A total number of about 23 species belonging to 12 different genera of fungi were isolated and identified. About 70% of the samples were infected with Aspergillus flavus and Aspergillus niger, with percentages of 33%, 40% recovered from maize and 46%, 27% recovered from rice, respectively. The percentages of infection of maize ranged from 16% to 142%. The percentages of infection of rice seeds ranged from 6% to 93%. Total aflatoxins and fumonisins detected in maize averaged 9.75 and 33 µg/kg, respectively. Total aflatoxins and fumonisins detected in rice averaged 5.15 and 1014 µg/kg, respectively.

Fusarium wilt of tomato (Lycopersicon esculentum) caused by Fusarium oxysporum f. sp. lycopersici is one of the most important diseases that affect this crop worldwide. This study aimed to biosynthesise nanosilver (AgNPs) using Chaetomium globosum, to evaluate its in vitro antifungal activity against pathogenic F. oxysporum and in vivo control of tomato seedlings wilt in the greenhouse. AgNPs was tested for its in vitro antifungal potential against F. oxysporum using poisoned food technique on three different growth media, agar well diffusion assay, inhibition of colony formation (CFU), and tested for its potency to control seedlings wilt upon its use at different concentrations (50, 100 and 500 mg/l) and for different incubation periods (0, 1, 2 and 4 h). Results indicated that C. globosum succeeded to biosynthesise AgNPs with maximum UV/vis absorbance around 420-450 nm, spherical in shape with particle size of 11-14 nm according to Transmittance electron microscope and displayed high purity recorded through X-ray diffraction (XRD). In vitro studies revealed high antifungal activity of AgNPs against F. oxysporum noticed especially at a concentration of 500 mg/l and after incubation period for 4 h. The CFU of F. oxysporum on potato dextrose agar (PDA) medium decreased significantly on increasing the concentration and time of incubation with AgNPs. In the greenhouse, AgNPs caused appreciable enhancement in the growth parameters of tomato seedlings such as; root, shoot fresh weight, and height of seedlings in soil infested with F. oxysporum compared with the control. In addition, AgNPs reduced the severity of wilt disease by 90% observed through decreasing the number of wilted seedlings especially after placing their roots in 500 mg/l of AgNPs suspension for 4 h prior to soil infestation with the pathogen. This study recorded for the first time that C. globosum has the ability to synthesise AgNPs which showed significant in vivo antifungal potential observed through control of Fusarium wilt of tomato seedlings, in addition to enhancing their growth parameters in the greenhouse.

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