Introduction: Now many studies conducted on the drug substance from nature that can serve as an anticancer agent as a potential chemoprevention agent, such as Annona muricata Linn leaf escort chemotherapy, which was flaring. The cancer cell in humans was included the loss of p53 protein function due to mutations in the protein gene. Other causes are that the p53 proteins are not functioning due to an increase in protein misfolding event chaperones and degradation events ubiquitous as binding by viral protein. Method: Cytotoxicity assay performed on 24 well plate micro-cultures. HeLa cells are as 2 × 10 4 cells in 100 mL in RPMI media. Created control is RPMI and solvent DMSO 0.25%. Cytotoxic Test performed by the method of calculation tryphan blue dye exclusion. Being fasted for 24 hours in the culture medium, then the cells are grown in micro-plate with media plus samples with a non-lethal concentration (LC50) of partition and fractionation Annona muricata Linn leaf. Sampling is performed at 24 hours. Each of these wells is calculated the number of living cells and made the curve of cell number and incubation time. Result: The results showed that HeLa cells are being LC50 partition of leaves Annona muricata Linn in ethyl acetate his cell death rate was higher (2000 µg/ml have 131.89%; 15.625 µg/ml have 11.37%) and in ethanol-distillate water his cell death rate was lower (2000 µg/ml have 35.80%; 15.625 µg/ml have 3.97%). Another results showed that HeLa cells are being LC50 fractionation of leaves Annona muricata Linn in chloroform his cell death rate was higher (2000 µg/ml have 91.86%; 15.625 µg/ml have 2.68%) and in ethyl acetate, his cell death rate was lower (2000 µg/ml have 23.79%; 15.625 µg/ml have 4.69%). Figure regression LC50 of HeLa cell culture treatment with partition or fractionation looks of regression test is the positive regression coefficient. Conclusion: Annona muricata Linn leaf in chloroform is a good candidate for chemoprevention escort chemotherapy for cancer causing viruses.

An in vivo study was carried out to investigate the role of tea, coffe and cigarette smoking upon the staining of teeth associated with the use of chlorhexidine gluconate. Three groups of volunteers, one of which consisted of cigarette smokers, rinsed with a 0.2% chlorhexidine gluconate mouthwash three times a day throughout two 10 day periods. The two non smoking groups were allocated tea and coffe respectively for consumptionduring on 10 day period. The smoking group were allocated coffee during one 10 day period. During the other 10 day periods the volunteers refrained from all hot beverages. A fourth group refrained from all hot bevrages during a 21 day period. The staining which developed on the previously cleaned teeth and tongues of the volnteers at the end of the respective rinsing periods was scored. All volunteers kept a diet record throughout the whole study. The drinking of tea and coffe significantly increased staining of teeth and tongue when compared with not drinking. Staining by tea was significantly worse than staining by coffee. Cigarette smoking appeard to have an additive effect on the staining. Dietary analysis indicated that other factors ar important to development of chlorhexidine staining and some apeared particularly chromogenic in this respect.

The Early-7 (E7) protein of HPV binds to the underphosphorelated form of the tumor suppressor protein – pRb and displaces the E2F transcription factor that is normally bound by pRb. The latent membrane protein-1 (LMP-1) of EBV prevents apoptosis of B cells by up regulating the expression of bcl-2, and it activates growth promoting pathway that are normally triggered by T cell – derivate signal. The aims of this study to know that in cervical cancer stay HPV and EBV.DNA was isolated from nineteen sample cervical cancer tissues frozen section. Diagnose related with HPV and EBV was made by Polymerase Chains Reaction (PCR).The result of this experiment showed that from 19 samples diagnosed as cervical cancer, 17 samples are positive HPV and 13 samples had HPV and EBV positive. The conclusion of this experiment is 89% of cervical cancers are infected with HPV and 68% also infected with HPV and EBV.

The aim of the study was to determine the effect of nutrition education in the form of DASH diet booklets on body mass index, waist circumference, mid-upper arm circumference, and blood pressure in obese adolescents. Methods: This study was quasi-experimental, which is pre-test and post-test with control group design. The number of subjects was 60 respondents. The groups in this study were divided into two; one treatment group and one control group. The first group was given a nutrition education intervention with a booklet and the second group was not treated. Data analysis used paired t-test and independent t-test (p=0.05; CI=95%), Wilcoxon test, Mann-Whitney test, and Spearman rank test. Results: There were differences in BMI, waist circumference, mid-upper arm circumference, and significant systolic blood pressure in the treatment group after the intervention. The mean average of the treatment group decreased significantly; BMI: 0.36±0.05 kg/m2; waist circumference: 0.84±0.14 cm; mid-upper arm circumference: 0.37±0.63 cm, and systolic blood pressure: 1.13±1.36 mmHg. In the control group the average BMI, waist circumference, mid-upper arm circumference, and systolic blood pressure were increased significantly. The treatment group experienced a decrease in energy intake by 524.65±85.77 kcal while physical activity increased by 0.635±0.09. Conversely, the control group experienced an increase in energy intake by 147.29±25.18 kcal, while physical activity decreased by 0.470±0.08. Changes in energy intake and physical activity between the treatment group and the control group were identified as significant correlations (p<0.001). Similar to it, the changes in the BMI, waist circumference, mid-upper arm circumference, and significant systolic blood pressure were identified as significant correlations (p<0.001). Application: The use of booklets can be a solution for the continuation of effective nutrition education as the nutritional information becomes easier to be comprehended and adapted by the booklet recipients and used in everyday life.

AIM: The study aimed to isolate and identification secondary metabolite from pericarp Garcinia mangostana Linn. METHODS: The first step of this research was maceration of sample using alcohol 70% solvent. The separation and purification of compounds using Vacuum Liquid Chromatography (VLC), Radial Chromatography (RC). The purity of isolate was analyzed by thin layer chromatography (TLC) and melting point. Compounds identified using spectroscopi IR, NMR-1D (1H, 13C-NMR and DEPT) and 2-D NMR (HMQC and HMBC). RESULTS: The compound has melting point at 165-167°C. The result showed isolate was gartanin. CONCLUSION: The secondary metabolite found in pericarp Garcinia mangostana Linn. is gartanin.