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The Mecardonia genus belongs to the Scrophulariaceae family, composed by 37 species, five of them original of Argentina. M. tenella is an attractive herbaceous native plant characterized by its intensive green foliage and their abundant ring shaped yellow flowers (Zuloaga and Morrone, 1999). It is a very interesting plant for pot and/or garden. A trait to improve in this species is the size of the flowers. To start a breeding program applying biotechnological approaches (plant transformation or in vitro mutagenesis) the establishment of in vitro micropropagation technique is required as the first step.

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In vitro germination of immature seeds of Jacaranda mimosifolia treated with gibberellic acid (GA 3 ) was studied. Immature seeds were collected monthly after crossings and sown on Murashige and Skoog (1962) medium with 3.0% sucrose and 0.6% agar after soaked 24 hours with 0, 10, 100, and 500 mg·L -1 GA 3 solutions. Though germination was observed in the immature seeds harvested 2 months after crossing (2 MAC), the rate was quite low. When immature seeds of 3 MAC treated with 100 or 500 mg·L -1 GA 3 solution were cultured, >60% germination were obtained within 2 weeks after culturing. These results indicate that immature seeds of 3 MAC treated with adequate GA 3 solutions, seedlings can be obtained precociously and the period from crossing to the seedling stage was shorter than for mature seeds.