Leishmaniases are a group of diseases caused by Leishmania protozoa affecting various vertebrate hosts, including humans and dogs. Dogs represent the most important domestic reservoir of leishmaniasis. For this reason, the aim of this study was to detect the presence of antibodies against Leishmania spp. in dogs being treated at the veterinary hospital of our institution. Samples were obtained from 175 animals by jugular or cephalic venipuncture, and serum was subsequently separated. Anti-Leishmania spp. IgG antibodies were detected in these specimens using indirect immunofluorescence. This revealed that of the 175 serum samples, those of four (2.28%) animals reacted positively and the antibodies title was 40. These data are consistent with the transmission of Leishmania spp. in the region, suggesting that dogs represent a reservoir of American cutaneous leishmaniasis. This is the first description of circulating antibodies against Leishmania spp. in the West region of Paraná State. Key words: Serological reaction. Leishmaniasis. Canine population. Toledo-PR. ResumoAs leishmanioses são doenças causadas pelo protozoário Leishmania e acometem vários hospedeiros vertebrados, incluindo humanos e cães. Cães representam o reservatório doméstico mais importante da leishmaniose. O objetivo do presente trabalho foi detectar a presença de anticorpos contra Leishmania spp. em cães atendidos na rotina de animais de companhia do Hospital Veterinário desta instituição de ensino. Amostras de sangue de 175 animais foram obtidas por venopunção jugular ou cefálica com posterior obtenção dos soros. Estas amostras foram submetidas à reação de imunofluorescência indireta para detecção de anticorpos da classe IgG anti-Leishmania. A reação sorológica dos 175 soros realizados revelou quatro (2,28%) animais reagentes com título 40. Estes dados revelam a presença de espécies de Leishmania spp. circulando na região, sugerindo o papel de cães como reservatórios para leishmaniose cutânea americana. Esta é a primeira descrição da circulação de anticorpos contra Leishmania spp. na região Oeste do Paraná. Palavras-chave: Reação sorológica. Leishmaniose. População canina. Toledo-PR.
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