Background:Zinc is an essential mineral that is found in almost every cell in the body. It is a key factor in making many parts of the reproductive system work properly. Copper play important role in male fertility it is essential element for the production of male gametes, substantial amounts of cu can also be found in liquids associated with sperm in the epididymis and prostate, also effect the integral androgen distribution in terms of fertility on the line hypothalamicpituitary-testis. Objective: To access the level of zinc and copper in serum among Sudanese male with abnormal semen analysis. Materials and Methods: This is a case control study carried out from Banoon fertility center in period from March to May 2018. We quantified the levels of Zn and Cu in serum samples from 50 patients of infertility and 50 healthy control people. These elements were determined using atomic absorption spectrophotometer. Results: Zn and Cu were significantly lower in infertile men patients, (0.269±0.093 mg/l) (0.223±0.084 mg/l) when compared with control group, (0.579±0.224mg/l) (0.821±0.179 mg/l), with P value (0.000) respectively.). Conclusion: There is a defect in levels of Zn and Cu in infertile men patients. The estimation of these trace elements could be a useful complementary in diagnosis and monitoring for infertility.