Context: Cancer is one of the leading causes of death throughout the world. Treatment failure in cancer can be caused due to drug resistance and toxicity. Therefore, it is necessary to develop new drugs that have relatively smaller side effects, one of which is by screening natural ingredients. One of the plants that have the potential for anticancer therapy is red betel (Piper crocatum). Aims: The purpose of this study was to determine the potential of methanolic extract of P. crocatum leaf (MEPcL) as a cytotoxic agent and phytochemical screening of MEPcL.