The present study describes the potential of mixed fungal isolates, i.e. Aspergillus niger, Penicillium chrysogenum and Rhizopus oryzae for the removal of zinc(II) and copper(II) from aquatic environments. Capacity of mixed fungal biomass to adsorb Zn(II) and Cu(II) were studied in batch sorption experiments as bioremediators. Optimal conditions from contact time, pH, initial metal ion concentration and temperature for remediation of Zn(II) and Cu(II) were studied. Typically, the uptake of Zn(II) and Cu(II) rises with increasing pH up to 4.0. Optimal metal concentration was 150 mg/l when the maximum removal of copper and zinc was 69.5% and 30.3% respectively, was observed at initial metal concentration. Maximum uptake for metals was achieved after 15 min. The maximum biosorption for copper and zinc by selected fungi was achieved at 7.0 g of biosorbent. IR spectrum of three fungal species showed the presence of C=O groups, amine and amide N-H functional stretch.Keywords: Biosorption, copper, electroplating effluent, fungal isolates, zinc.WASTEWATER during electroplating activities involves poisonous heavy metals like chromium, mercury, zinc, nickel, cadmium, phosphorus and copper which build up in the food chain and cause different pathological states 1,2 . In recent years, association of different microbes has been used for wastewater treatment as bioremediators, as it was considered to have greater capability over pure and single isolates. The axenic cultures were devised to be mineralized rapidly and completely by the metabolic activity of different microorganisms 3 . The interactions between different heavy metals and microorganisms might be due to antagonistic, additive effects or synergic. These interactions might be multifaceted and distinguished which depends on the type of heavy metal ion and microbial species consortium. The collective effect of different heavy metals may be toxicity or growth stimulation in the same microbial consortium assorted from the additive effect of the single metal ions 4 . Fungi have diverse mechanisms for bioremediation of heavy metals, including metal uptake by cell-wall components, accumulation inside the cell or extracellular chelation by phytochelatins and metal lothioneins 5 . Waste samples were collected from a polluted water source near Al-Madina electroplating industry, Alipur Chattha, Pakistan, in sterilized and cleaned 1 l glass bottles with stopper. All samples were treated with 372 mg/l EDTA as the chelating agent to reduce toxicity in samples burdened with heavy metals. During examination, the samples were fresh, and were occurred immediately after return to the laboratory.The fungi were isolated by filtration of the electroplating waste for collection of fungal propagules using 0.45 m pore size filter paper 6 . The fungal spore suspension was prepared in 50 ml of saline solution, and 100 l of spore suspension was inoculated in sterilized petri dish with modified culture medium consisting of aureomycin (0.35 mg/l), glucose (10 g/l), rose bengal (0.035 mg/l), Cu...