The new antigen formulation was able to stimulate and expand the lymphoid constituents of spleen tissue. The SWP is where immune responses and antibodies are produced. Therefore, the effect of the antigen preparations on secondary immune responses, adaptive immunity, and antibody production is important in determining the susceptibility of mice to cutaneous Leishmaniasis and the induction of immunity.

In this study, the LBT Group has statistical differences regarding IL-12 and IL-10 from the other Groups.

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