Urinary Tract Infections (UTIS) are the most common type of healthcare-associated infection reported, among UTIS acquired in the hospital, approximately 75% are associatessd to urinary catheter. The aim of this study is to determine the attitude and practice of nurses toward prevention of Catheter Associated Urinary Tract Infection (CAUTI). A cross sectional survey design was used to select the sample of 148 Nurses. The data were collected using self-structured questionnaire and analyzed using SPSS version 16.0. The result shows that most of the respondents are males (59.1%) within the age range of 31-40years (56.9%) and with working experience of >3years (70.1%). The attitude of the respondent toward CAUTI prevention was good; practice was very good with more than 80%, catheter care was very good also with 84.7% agree that closed drainage system will prevent CAUTI. In conclusion the respondents shows good attitude and practice toward prevention of CAUTI.