Introduction: Helicobacter pylori (H. pylori) is a spiral-shaped, Gram-negative bacterium that affects up to 50% of the population worldwide. In our study, we aim to determine the level of awareness of the students of the Syrian Private University about this germ. Methods: A cross-sectional study in which a knowledge questionnaire was relied upon that included asking about a group of personal information and measuring knowledge of H. pylori, and this knowledge was tested with appropriate statistical tests. Results: Our study shows that there is average knowledge among university students about H. pylori, and their information is closely related to previous knowledge of the bacterium, the academic year, and sources of knowledge.