AMPLES of water, sediments and aquatic plants were collected from different sites along EL-Gharbia main drain. The sampling processes were carried out seasonally. The collected samples were subjected for a series of analyses, in terms of total and available contents of macro nutrients and potentially toxic elements (PTEs), biological investigation (pathogens contents) and chemical oxygen demand (COD) and biological oxygen demand (BOD). Water samples taken at most of the study sites during the year can be regarded acceptable for agricultural use, but only under particular conditions. On the other hand, these waters are not appropriate for agricultural use from a biological point of view. Enrichment factor (EF) values of the sediments were low for Mn, Zn, Co, B, Co, Cr, Ni and Pb in all seasons and at most sites. Unlike the EF values of the aforementioned PTEs, the enrichment factor for cadmium was generally, high at most sites within the four seasons. EF values of Pb were moderate at most sites. The bioaccumulation factor (BCF) values of the shoot of aquatic plants were generally, high for all elements in all seasons; except for Mn and Pb in summer and winter seasons at some sites, Zn in winter season at some sites, Cu and Ni in all seasons at some sites, and Co in autumn and winter seasons. The BCF values of the roots were high for all the elements in all seasons; except for Cu in the summer and winter seasons at some sites, Zn in the winter season at some sites, and Pb in summer season at some sites. Principal component analysis (PCA) revealed that these metals were originated from natural (Cd, Co, B and Cu) and anthropogenic (Fe, Zn, Mn, Cu, Ni and Cr) sources.
