Hospitals are the centers of taking care of public health. However at the same time they generate hazardous biomedical waste. Hospitals in Pakistan generates about 0.8 million tons of waste every day and approximately 250,000 tons of waste per year. Its mismanagement results in transmission of diseases, major environmental threats and occupational hazards. A descriptive cross-sectional study was carried out among health care professionals. Sample was collected by using multistage random sampling. Among all participants studied mean age was (29.56 ± 7.59). Majority of the respondents had good knowledge (64.7%), OT department had good knowledge (26%) while knowledge of the nurses were (33%). Over all Confident attitude of the respondents were (53%), respondents from OT department had confident attitude (28%) while nursing staff had confident attitude of (39%). Overall good practices of the respondents were (65%), OT department had good practices (30%) whereas nursing staff had also good practices (41%). The relationship between experience and attitude, awareness and practices, and attitude and practices was found to be statistically relevant at (p = 0.05). Our study concluded that knowledge and practices scores of health care professional was satisfactory but the attitude of health care professionals was somehow low. The satisfactory score of knowledge, attitude and practices was observed higher amongst nurses compared to other healthcare professionals; this may be attributed to increased interest in biomedical waste management.