Pseudomonas aeruginosa, a Gram-negative human pathogen, P. aeruginosa is lone of the furthermost common hospital pathogens also is a chief concern, particularly in immune-compromised patients. The purpose of this study was to identify phenotypic and genotypic antibiotic resistance in Pseudomonas aeruginosa isolated from wound infection The bacterial isolates (30) were obtained from patients admitted to Mirjan Medical City in Babylon, Iraq (burns, wound unit). was identified biochemically and morphologically, and the isolates were subjected to standard bacteriological culturing processes on blood and MacConkey agar plates for 24-48 hours at 37oC for isolation and purification, Viteck 2 compact system confirmed the isolates and antibacterial sensitivity as well. These findings revealed that P.aeruginosa has a high rate of penicillin resistance, with a resistance rate of (100percent ) isolates. Advanced resistance to cephalosporin antibiotics was also found in resistant isolates of Cefoxitin, Ceftriaxone (75%), Ceftazidime, and cefepime (85%). For carbapenem antibiotics, had a high resistance rate (90percent). Aminoglycosides have variable resistance to Amikacin (60percent), Gentamicin (70 percent), and tobramycine resistant (90%). The findings revealed that all Pseudomonas aeruginosa isolates tested positive for Class 1,2 Integron resistance genes. with positive results (35%) for Int1 and 35% for Int2 (25%). This study found that P.aeruginosa has a high rate of resistance to Penicillins, Cephalosporin, Carbapenem and Aminoglycosides antibiotics.

This study aimed to assess antibiotics resistance and antibacterial action of silver nanoparticles against Staphylococcus aureus isolated from wound infection. A total of 100 samples of wound swab existed calm since wound patients who stayed the Al-Hillah, Teaching Hospital (wound unit) in, Babylon province, Iraq. S aureus was recognized biochemically and morphologically. A whole of 30(30%) of the whole specimens tested confident for S.aureus. Available of 30 S.aureus isolates, 8(26.6 percent) were MRSA. Antibiotic susceptibility for 8 antibiotics for MRSA that appeared to Penicillin G and Cefoxitin was tested, and all isolates were resistant (100percent), Were susceptible to Rifampin, Tetracycline, and Ciprofloxacin (62.5percent) Clindamycin sensitivity remained experimental in 75% isolates. Resistance to Erythromycin remained establish in approximately 62.5 percent of the population. Gentamycin resistance was found in 50% of the isolates. The antibacterial activity of silver nanoparticles (AgNPs) alongside S.aureus demonstrates extreme broad-range antibacterial act in contradiction of recognized bacteria, with an rise inhibition zone diameter related to nanoparticle concentration The MIC of Ag NPs ranged from 50 to 100g/ml, while the MBC ranged from 100 to 200g/ml. Ag NPs is suggested as an effective anti-MRSA alternative. This experiment discovered that Ag NPs is highly recommended as an alternative anti-MRSA agent with significant inhibitory and antibacterial effect due to the methicillin resistant strains' high rate of resistance to Penicillin G and Cefoxitin (100%), Erythromycin (62.5%), and Gentamycin resistance (50%).