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An in vitro investigation was conducted to evaluate the potential role of exogenously applied ascorbic acid in alleviating the effect of sodium chloride (NaCl) in two varieties of rice, Oryza sativa L. (MRQ74 and MR269), that differ in salt tolerance susceptibility. Seven-day-old rice seedlings germinated on Murashige and Skooge (MS) medium were transferred to MS media containing (200 mM) NaCl and (0, 0.5, 1, 1.5 mM) ascorbic acid for 14 days. The results showed that both varieties of seedling, when exposed to salt stress, exhibited a significant reduction in N, K + , chlorophyll and growth characteristics, fresh weight, dry weight and the lengths of shoot and root. However, the exogenous ascorbic acid in the medium led to an improvement and amelioration of the NaCl stress.

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Abstract-Inter simple sequence repeats (ISSRs) have been utilized widely for molecular markers in analyzing the genetic diversity and phylogenetic and regions in the genome flanked by microsatellite sequences. PCR amplification of these regions using a single primer yields multiple amplification products that can be used as a dominant multilocus marker system for the study of genetic variation in various organisms. For this study provides, DNA isolation, adjusting in six factors (Buffer, MgCl 2 , dNTPs, ISSR primers, Template DNA and Taq polymerase) at six levels, and optimization of PCR temperature for the ISSR reaction was 60-45 °C, primers screening on indica rice (Oryza sativa). In this research, simple method of DNA isolation by using seedling. The objective of the present investigation was to assess the optimizations and quantification. Has been shown that stalk enhanced the maximum value of genomic. The results show that 100 ISSR primers were examined as well as, 56 ISSR primers was productively amplified. Optimum components for PCR reactions were 5.0 μl of 5X PCR Buffer, 1.5 μl of 25mM MgCl 2 , 1 μl of 10 mM dNTP, 1 μl of 10 Μm ISSR primers, 2 μl Template DNA, and 0.1 μl of 5 units/ml Taq polymerase. Based on this study, has brought out some information on the relationship between these ISSR primers will be applied further for molecular profiling as well as response evaluation in rice varieties.