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The objective of this research was to investigate the effect of flamboyant flower extract as a natural antioxidant on sperm quality of Aceh cattle after dilution and equilibration. Fresh semen was collected from 2 healthy bull aged 3.5-4 years old. The research took place in BIB Laboratory. The method used was CRD (Completed Randomized Design), with 6 treatments and 5 replications each The treatments (m/v) were 0%, 0.05%, 0.10%, 0.15% and 0.20% of flamboyant flower extracts. The method used was CRD (Completed Randomized Design), with 6 treatments and 5 replications each. The treatments (m/v) were 0%, 0.05%, 0.10%, 0.15% and 0.20% of flamboyant flower extracts. The data obtained were analyzed by analysis of variance (ANOVA). Parameters evaluated in this study were motility, viability, plasma membrane integrity, and abnormality. Results showed that there was an insignificant effect (p>0.05) of flamboyant flower extract on the quality of Aceh cattle spermatozoa. The best percentage of motility and viability, 80.0% and 88.4%, respectively, were found at P3 (0.15% flamboyant flower extract). In can be concluded from the research that the addition of 0.15% (m/v) D. regia flower extract was able to maintain sperm quality of Aceh cattle to be used in the artificial insemination program.

Birds are organisms that can be found in a variety of habitats. One of the bird habitats is paddy fields. Birds found in paddy fields generally carry out activities such as foraging, breeding, and competing. This study aims to determine the diversity of birds in paddy field habitats during the paddy ripening phase (Oryza sativa L.). The study was conducted from April to June 2022. The location of the study was Jurong Peujera Village, Ingin Jaya District, Aceh Besar Regency. This study used the Point Count method with a radius of 50 m and a distance between points of 150 m. Observations were made at 9 observation points with 4 repetitions, in which the duration of observations was 10 minutes at each observation point. The observation was conducted in the morning and evening at 06.30-10.00 AM and 03.30-05.00 PM. The results obtained were 2586 bird individuals consisting of 14 species from 11 families. The species with the highest number of individuals are Lonchura punctulata with 1535 individuals and Bubulcus ibis with 637 individuals. The highest percentage of families by species is Ardeidae (22%), and Estrildidae (15%). The Shannon-Wiener bird species diversity index in the paddy field area during paddy ripening phase was obtained H'=1.24 which indicates the level of diversity of the bird species is moderate. Meanwhile, based on the dominance index, Simpson shows a value of C=0.42 which indicates a low bird dominance level. The low level of bird dominance is due to the moderate diversity of species in the paddy field area.

The propose of these research was to prepare monoacilglyserol (Ethanolysis Product from Ketapang Seed Oil/EPKSO) by ethanolysis reaction and to measured it’s antibacterial activity as free materials and as immobilizes material in starch film/patch. The oil of ketapang seed was extracted by pressing method. Fatty acid in the oil was identified with Gas Chromatography (GC). Ethanolysis process used 5 mL ketapang seed oil and 2mL NaOH in 100 mL ethanol 95%. Sample EPKSO from ethanolysis of ketapang seed oil were compare to EPKSO standard from SEAFAST Centre. The preparative TLC of ethanolysis product was eluted with n-hexane: diethyleter: formic acid (80:20:2) v/v. EPKSO was produced by TLC preparative from ethanolysis product. Antibacterial activity of EPKSO from ethanolysis reaction was assayed against Streptococcus mutans with diffusion method. From this research, ketapang seed oil obtained was 7.69 % w/w. The major fatty acid from ketapang seed oil from Gas Chromatography (GC) were palmitic acid, stearic acid, oleic acid and linoleic acid. The minor of fatty acid were miristic acid, palmitoleic acid, heptadecanoic acid, cis-10-heptadecanoic acid, belaidic acid, arachidonic acid, cis-11-eicosenoic acid, linoleic acid, cis-11.14-eicosedienoic acid, behenic acid, trisanoic acid and lignoseric acid. Both of the EPKSO have similar Rf (0.07). The amount of EPKSO result from ethanolysis ketapang seed oil was 1.79 % w/w. This EPKSO can inhibit S. mutans at 0.4 % w/v with inhibition zone 183 mm2. EPKSO was incorporated in starch based film/mucoadhesive patch with variation 2 MIC, 4 MIC 6 MIC dan 8 MIC. After incorporated with EPKSO starch based film/mucoadhesive patch can inhibit the growth of Streptococcus mutans at all EPKSO concentration in film/patch. Most of mechanical properties of film/patch still meet the range of standard. Organoleptic assay showed no significant difference between starch film with and without EPKSO.