This study was carried out in the Center of Endocrinology and Diabetes in Baghdad during the period between October 2019 to February 2020. The aim was to measure the level of some apoptosis markers and some autoimmune antibodies related to the thyroid gland in Iraqi patients with hyperthyroidism and evaluate the correlation between all the measured parameters. The study included 88 patients who were divided into three groups; group 1 included 30 newly diagnosed hyperthyroidism patients (24 females, 6 males); group 2 included  30 patients of hyperthyroidism who were under treatment (28, 2 males); group 3 included 28 healthy individuals as control group (22 females, 6 males).      Most of the patient's ages ranged between 40 to 60 years (73.3%), while60.7% of the control group were within the same age category. The highest rate of disease was in females compared with males (86.7% vs. 13.3%). The current study included 30% of newly diagnosed hyperthyroid patients and 30% of patients undergoing treatment for a while. The majority of the hyperthyroidism patients, both newly diagnosed and treated, were overweight, and they accounted for 53.3% of each group.    Highly significant differences (p=0.001) were found in the level of TNF-α in the newly diagnosed and under treatment patient groups in comparison with the level in the control group. The results show a significant decrease in TNF-α level in the treated patients as compared to its levels in the other groups, which indicates that this factor is affected by the given therapy.    It was found that 25% of the patients with hyperthyroidism were suffering from diabetes, with a significant correlation (p=0.009) between hyperthyroidism and diabetes mellitus. It was observed that these patients have a significant increase (p=0.038) in the level of p53 as compared to its level in patients with non-diabetic hyperthyroidism patients and healthy subjects.     This study shows a non-significant negative correlation between TNF-α and TSH levels (r= -0.06) and a non-significant positive correlation between TNF-α and p53 levels (r= 0.17) in hyperthyroidism patients.      The positive correlations between some apoptosis markers and anti-TSHR antibodies and between TSH and these antibodies in hyperthyroidism patients refers to an increase in the concentration of apoptosis markers, which may lead to an increase in the levels of thyroid autoantibodies, which affects thyroid tissue potency and increases thyroid hormone production.

Background: Infective keratitis is the most common cause of blindness and preventable ocular morbidity worldwide. There are many published series of infective keratitis from both temperate and tropical parts of the world, and management strategies are well established1.Objective: The current study was aimed to detect the specific bacteria and predisposing factors that predisposed for the bacterial keratitis.Material and methods: Retrospective study of the hospital records of 40 patients who were diagnosed as bacterial keratitis and treated at the Ophthalmology in-patient department of at Ibn Al- haithum Teaching Hospital from May 2015 to December 2015.Patients who don’t have corneal scraping, or culture and sensitivity findings discarded from this study. Predisposing factors, clinical and microbiological data were reviewed. Corneal scrapings were obtained by physicians then were subjected for bacterial culture and biochemical tests.Results: Pseudomonas aeruginosa was the most common bacteria isolated at higher percentage 19(47.5%) cases whereas Klebsiella pneumoniae isolated at lower percentage 2(5%). The most common risk factors was foreign body in 10 (25%) followed by contact lenses in 8 (20%) patients.Conclusion: Gram negative bacteria were the most frequent bacterial organisms especially Pseudomonas aeruginosa isolated from corneal scraping and corneal ulcer was found to be occurring principally by foreign body followed by contact lens wearing.

Background: Infective keratitis is the most common cause of blindness and preventable ocular morbidity worldwide. There are many published series of infective keratitis from both temperate and tropical parts of the world, and management strategies are well established1. Objective: The current study was aimed to detect the specific bacteria and predisposing factors that predisposed for the bacterial keratitis. Material and methods: Retrospective study of the hospital records of 40 patients who were diagnosed as bacterial keratitis and treated at the Ophthalmology in-patient department of at Ibn Al-haithum Teaching Hospital from May 2015 to December 2015.Patients who don't have corneal scraping, or culture and sensitivity findings discarded from this study. Predisposing factors, clinical and microbiological data were reviewed. Corneal scrapings were obtained by physicians then were subjected for bacterial culture and biochemical tests. Results: Pseudomonas aeruginosa was the most common bacteria isolated at higher percentage 19(47.5%) cases whereas Klebsiella pneumoniae isolated at lower percentage 2(5%). The most common risk factors was foreign body in 10 (25%) followed by contact lenses in 8 (20%) patients. Conclusion: Gram negative bacteria were the most frequent bacterial organisms especially Pseudomonas aeruginosa isolated from corneal scraping and corneal ulcer was found to be occurring principally by foreign body followed by contact lens wearing.

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The research was aimed to demonstrate the frequency of virulence factors genes (exoenzyme S and exotoxin A) and to express their relationship to antibacterial resistance among Pseudomonas aeruginosa isolated from patients suffering from otitis externa. The results were revealed that only twenty one (32%) out of 65 clinical ear swabs were Pseudomonas aeruginosa. According to PCR amplification, 18 (85.7%) bacterial isolates were expressed both toxins ( exoenzyme S and exotoxin A). The data get by real time experiments were revealed that the isolates were give higher percentage of resistance were seen against Ceftazidime (90.5%) and Gentamicin (88.5%).Only thirteen isolates (61,90%) of Multidrug resistance (MDR) –Pseudomonas aeruginosa isolates were detected, and two (9.5%) isolates were resist to all 8 classes of antibiotics and considered as PDR, and three (14.2%) bacterial isolates resist seven classes of antibiotics which considered as XDR.