АbstractAim: Design a new pharmaceutical technology for isolation novel ecdysteroid 3α,14α,22R, 25-tetrahydroxy-5α (Н)-cholest-7-en-6-one from the epigeal organs of ComastomatenellaRottb. Research NMR spectrscopy and biological activity. Material and Methods: The isolation procedure involved extraction with aqueous ethanol, partitioning(petroleum ether-ethyl acetate) of the obtained extract to remove non-polar impurities, extraction of the water layer with isobutyl alcohol, and subsequent purification by aluminum oxide column chromatography. According to NMR spectroscopy, mass spectrometry, and microanalysis, the structure of 3α,14α,22R,25-tetrahydroxy-5α(Н)-cholest-7-en-6-one (1). Results and Discussion: During the study of plants ComastomatenellaRottb was isolated new phytoecdysteroid.According to NMR spectroscopy, mass spectrometry, and microanalysis, the structure of 3α,14α,22R,25-tetrahydroxy-5α(Н)-cholest-7-en-6-one.Isolated object was examined for anti-inflammatory activity of the compound.Thus, the present study established high anti-inflammatory activity of 3α,14α,22R,25-tetrahydroxy-5α(Н)-cholest-7-en-6-one1 in the dose of 50 mg/kg in an experimental model of an acute exudative reaction. Conclusion: From the results of the study on the anti-inflammatory activity, it became clear that object potently and significantly reduced the number of abdominal writhings as compared to the control animals.