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In the article, the analysis of the introduction of innovative technologies which are applied in profiling disciplines within the framework of the training experts on “medical-preventive business” is given. There are presented areas of subject-specific competencies, which are introduced and summarized in clusters. The effectiveness of pedagogical technologies promoting the educational and cognitive activity of students in the field of sanitary and hygienic monitoring is shown. For the creative use of subject knowledge and skills, there are widely used non-imitative active research methods allowing students not only realizing personally themselves in the performance of research work but also studying in detail scientific research problems in the field of hygiene. There is presented the experience of the use of applied computer technologies, allowing students developing virtually practical skills and skills of conducting hygienic monitoring on the simulators. Applied modules enable students to develop the necessary practical skills of sanitary supervision, demonstrate the search, analytical and practical stages of work, taking into account all indices and environmental factors, with following generalization and systematization of the conclusions of their own research. Training is accompanied by the active participation of students in the scientific activities of the department, with the preparation of scientific reports and theses, and work in a scientific student group. Practical skills of working with hygienic equipment are acquired by students according to algorithms and standards, specifically for each device and allow to unify their actions when measuring environmental factors and production environment. The applied pedagogical technologies in the training of future hygienists are directed more towards the development of independent thinking, have a reasoned position on how to solve the problem, work in a team and find the most rational solution to the hygienic task