Objective: The purpose of this research was to evaluate the antibiotic resistance profile of urinary tract infection-causing bacteria and their prevalence among children presenting to a tertiary care hospital. Study Design: Cross-sectional/ Descriptive study Place and Duration: This study was carried out at Khyber Teaching Hospital, Peshawar from June, 2021 to May 2022. Methods: Total 112 suspected cases (children) of UTI with ages 6-months to 14 years were presented. After receiving parental agreement, we documented the children's age, gender, residence, and parental education level.It was decided to perform a urine dipstick test to check for nitrites and leucocytes, and then analyze the leucocytes and bacteria under the microscope. Antimicrobial resistance was tested using the modified Kirby-Bauer disc diffusion technique with a panel of antimicrobials.SPSS 20.0 was used to analyze all data. Results: Among 112 included cases, 58 (51.8%) were males and the rest were 54 (48.2%) female children. Frequency of leucocytes and nitrites by urine dipstick method was 30 (26.8%) and 12 (10%) but under the microscope, number of leucocytes were 15 (13.4%) and bacteria was 9 (8.03%). There were 65 (58.04%) gram positive and majority were females and 47 (41.96%) were gram negative cases. We found E.coli was the most prevalent microorganism found in 46 (70.8%) cases, 5 (7.7%) Klebsiella spp. and Pseudomonas spp. was 4 (6.2%). We found that microorganism were highly resistant (100%) to antibiotics ceftazidime, cefoperazone, ampicillin and ofloxacin while ciprofloxacin, cefuroxime and ceftriaxone showed high sensitivity. Conclusion: We observed that E.coli was the most frequent bacteria in UTI urine samples. Antimicrobial resistance in UTI-causing pathogens is worrying. It's crucial to monitor UTI-causing microbes' antibiotic susceptibility trends. Keywords:UTI, E.coli, Klebsiella spp., Resistance, Sensitivity

Objective: The primary aim of this research was to study the molecular history of fibroblast growth factor 21 (FGF21) as well as its physiological and pathological functions. Study Design: Observational study Place and Duration: This study was carried out at Hayatabad Medical Complex from December 2021 to April 2022 Methods: There were 77 patients of non-alcoholic fatty disease had age 20-65 years were presented in this study. After obtaining informed written consent details demographics were recorded. Diseases of all the patients were recorded and their relation with the FGF21 levels was observed. SPSS 23.0 was used to analyze all data. Mean standard deviation was use for categorical variables. Results: There were 42 (54.5%) males and 35 (45.5%) females among all cases. Mean age of the patients was 40.11±8.74 years and had mean BMI 27.8±11.34 kg/m2. Majority of the patients 31 (40.3%) had diabetes mellitus, 24 (31.2%) cases had renal failure, 20 (25.97%) cases had cardiovascular disease, mitochondrial disease in 17 (22.1%) cases, energy metabolism disorder in 15 (19.5%), lipid metabolism disorder in 13 (16.9%) and frequency of stroke was 9 (11.7%). We found significantly increased volume of FGF21 among diabetic obese cases which were resistant to insulin with p value <0.005. Conclusion: Pathophysiological functions, potential risk factors, and diagnostic biomarkers for endocrine FGFs in adult metabolic and genetic diseases are all supported by the available evidence. Pharmaceutical research is being conducted on endocrine FGFs. These results provide insight on the pathological and physiological functions of endocrine FGFs and offer new insights into the diagnosis and treatment of metabolic disorders. Keywords: FGF21, Lipid Metabolism, Pathaphysiology, Outcomes

Introduction: Since plasma microRNAs (miRNAs) are stable in the circulatory system and are associated with a number of disorders, including coronary artery disease (CAD), the primary worldwide source of mortality and morbidity, they have been found in these conditions. Objective: This study aimed to define the functions of miRNAs in lipoprotein and lipid metabolism with a focus on the conceivable participation in the onset and development of atherosclerosis. Methods: This study was carried out at Hayatabad Medical Complex from November 2021 to March 2022. 85 patients (25 females and 60 males) diagnosed with the coronary angiography (CAG) were included because they were suspected of having CAD. Results: The results showed that patients with CAD exhibited down-regulated expression of miRNA-199a-5`, miRNA-135a-3`, miR17-5`, and miRNA-222-3` and up-regulated expression of miRNA-144-3`, miRNA-185-5`, miRNA-133a-5`, and miRNA-222-5` in comparison to the control group. Individuals with CAD and the group that didn't take statins both had significantly higher levels of miRNA-144-3` than the control group (p=0.040 and p=0.018, respectively). Both the statin and non-statin groups significantly outperformed the control group in terms of plasma level of miRNA-133a-5` (p=0.037 and p=0.011, respectively). Conclusion: The current study's findings revealed that CAD patients' expression levels of the miRNAs HSA miRNA-144-3`, HSA miRNA-222-5`, and HSA miRNA-133a-5` are significantly different from controls. Keywords: microRNAs, lipid metabolism, atherosclerosis, treatment

Introduction: The diagnosis of pregnancy from forensic bloodstains can be useful in cases of infanticide, criminal abortions and missing person identification. Objective: This research illustrated the use of a rapid, precise, and tremendously responsive enzyme immunoassay kit designed for medical usage, which is put to good use in our lab for qualitative HCG detection in blood stains and has proven to be a useful tool in forensic pregnancy identification. Methods: HCG concentrations had previously been generally known, and total eighty whole blood samples were taken: forty expectant females (every single one between months one to six of pregnancy), twenty healthy young males, and twenty postmenopausal females with good health ration has been cleared in all data. Results: Enzyme immunoassay is a useful forensic technique for detecting human chorionic gonadotropin hormone in pregnant women. In the forty sample batch that was dated for six months, 37 samples (92.5%) yielded positive results, 38 of which (95%) yielded good outcomes in the qualitative analysis, most of them within the sensitivities boundary. The samples that were diluted to 1/100 and 1/200 and kept at room temperature for one week and six months period, respectively, produced just two (5.0%) and five (12.5%) successful results and the samples showed negative results when diluted to 1/200 and stored for six months. Practical implication This paper reports on human chorionic gonadotropin (HCG) detection in bloodstains based on enzyme immunoassay. Conclusion: Enzyme immunoassay has proven to be a suitable method intended for detecting an hCG hormone in blood stains, allowing for the qualitative assessment of hCG, and making it particularly interesting for use in forensic science applications. Keywords: Enzyme immunoassay, human chorionic gonadotropin (hCG), pregnancy, bloodstain

Introduction: Diabetes mellitus (DM) is a chronic metabolic disorder that requires a multifaceted approach to manage the disease, including lifestyle changes, medication, and monitoring blood glucose levels. Zinc is an essential micronutrient that plays a critical role in regulating insulin secretion, glucose metabolism, and immune function. Zinc supplementation may improve glycemic control and reduce oxidative stress in people with type 2 DM. Objectives: To determine whether zinc supplementation can enhance glycemic control in type 2 diabetic patients by investigating the relationship between serum zinc levels and glycemic control. Methodology: 100 participants with type 2 DM were recruited from local DM clinics and hospitals to participate in the study. Fifty participants were assigned to the zinc supplementation group and fifty to the placebo group. At the beginning and conclusion of the 12-week study period, serum zinc, HbA1c, and fasting plasma glucose levels were measured using blood samples. Using the t-test and regression analysis, SPSS (version 23.0) was used to conduct statistical analysis. Results: The mean age of the participants was 58.2 years, and the majority had a BMI greater than 25. There were no significant differences between the two categories in terms of participant characteristics.