BACKGROUND: Periodontal disease is associated with colonization of specific bacteria in the subgingival area. Widespread use of antimicrobials has resulted in emergence of various side effects and drug resistance. Phytomedicine has been used in many clinical conditions and it appears to be a suitable alternative to manage conditions affecting the oral cavity. AIM: Present study aims to assess the in-vitro antimicrobial efficacy of Tulsi extract and Curcumin extract on Periodontal pathogens. MATERIALS & METHODS: Ethanolic extract of 100% w/v of Tulsi and Curcumin was prepared by cold extraction technique. Prepared extracts were further diluted to obtain solutions of 5 different concentrations to be tested (0.5%, 1%, 2% and 5%). Obtained solutions and controls (0.2% Chlorhexidine and Distilled water) were subjected to the microbiological investigation against Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. Agar well diffusion method was employed to determine the concentration at which Tulsi and Curcumin gave an inhibition zone, similar to Chlorhexidine. Data was analysed using one‑way analysis of variance and post‑hoc test for inter-group and intra‑group comparisons. RESULTS: At 10% concentration both Tulsi and Curcumin extract have demonstrated comparable antimicrobial activity against Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis.

The aim of the study was to compare and evaluate the effect of diode laser as an adjunct to scaling and root planing and scaling and root planing alone on clinical parameters and Gingival Crevicular Fluid (GCF) interlukin-10 level in chronic periodontitis. Ten chronic periodontitis patients were selected. As it was a split mouth study, two sites with deepest pocket one in each quadrant were randomly assigned for scaling and root planing (SRP) and SRP+ diode laser treatment. Plaque index, gingival index, probing depth, and clinical attachment level were measured at baseline and at 3months after treatment. The GCF levels of interleukin-10 (IL-10) were analyzed by enzyme-linked immunosorbent assay. Test group showed significantly better outcome compared to the control group in all clinical parameters (PI, GI, PD and CAL). The total amount of IL-10 increased after treatment in both test and control groups. From baseline to three months, the SRP with Diode laser group demonstrated a substantial rise in IL-10 levels, demonstrating how lasers can improve nonsurgical periodontal treatment results. Thus, diode laser when used in adjunct to SRP, the levels of IL-10 which is an anti-inflammatory cytokine, significantly increased which indicated reduced inflammation of the tissues.

Aim: To evaluate the clinical efficacy of subgingivally delivered SMV and Placebo gel and compare in the treatment of stage II periodontitis when used as an adjunct to SRP and their antimicrobial effect on the Porphyromonas gingivalis bacteria. Materials & Methods: In a split mouth study design, 10 systemically healthy patients with stage II periodontitis were included in this study based on the inclusion and exclusion criteria. Two sites with deepest pockets were selected. Plaque samples were collected from these pockets at baseline followed by full mouth scaling and root planning, In Group I 1,2% SMV gel and in Group II Placebo gel was placed in the subgingival pockets. Microbiological analysis for Colony Forming Units of P.g was done at baseline after 3 months, Clinical parameters (GI, PI, PD, CAL, mSBI) were evaluated at baseline (before SRP), 1 month and 3 months. Result: Both the groups showed significant reduction in GI, PI, PD, CAL, mSBI and CFU of Pg but Group I showed more significant reduction than Group II.