The current study aimed to determine the prevalence of class A genes in the Extended Spectrum of β-Lactamase producing E. coli and K. pneumoniae isolated from Patients Urine Samples in United Arab Emirates. A total of 3457 urine samples were collected from October 2018 to January 2019. ESBL screening and confirmatory test were done using MicroScan WalkAway automated bacterial identification system. The ESBL class A gene primers were used to detect the blaCTX-M, blaSHV & blaTEM. Out of the collected urine samples, 474 were reported positive for urinary tract infection. A total of 130 samples were identified as Gram negative ESBL producing species, Escherichia coli (n=107) and Klebsiella pneumoniae (n=23). Amid these 130 ESBL producers, the class A ESBL genes blaTEM, blaCTX-M & blaSHV were detected in 78 isolates of which 59 were E. coli and 19 K. pneumoniae. In the identified 59 E. coli isolates, the most predominant gene was blaTEM, followed by blaCTX-M and blaSHV, and these three genes were detected together in 02 E. coli. In 10 E. coli and 11 K. pneumoniae two class A genes were detected in combination of blaCTX-M + blaSHV, blaSHV + blaTEM and blaTEM + blaCTX-M.