No abstract

Expandable graphite (EG) and few-layer graphene (FLG) have proven to be instrumental materials for various applications. The production of EG and FLG has been limited to batch processes using numerous intercalating agents, especially organic acids. In this study, a Taylor−Couette reactor (TCR) setup is used to expand and exfoliate natural graphite and produce a mixture of EG and FLG in aqueous solutions using an amphiphilic dispersant and a semiflexible stabilizer. Laminar Couette flow structure and high shear rates are achieved via the rotation of the outer cylinder while the inner cylinder is still, which circumvents vortex formation because of the suppression of centrifugal forces. Our results reveal that the level of expansion and exfoliation using an aqueous solution and a TCR is comparable to that using commercial EG (CEG) synthesized by intercalating sulfuric acid. More importantly, the resultant EG and FLG flakes are more structurally homogeneous than CEG, the ratio of FLG to EG increases with increasing shearing time, and the produced FLG sheets exhibit large lateral dimensions (>10 μm). The aqueous solutions of EG and FLG are wet-spun to produce ultralight fibers with a bulk density of 0.35 g/cm 3 . These graphene fibers exhibit a mechanical strength of 0.5 GPa without any modification or thermal treatment, which offers great potential in light-weight composite applications.

Regeneration of the central and peripheral nervous system is challenging since the functional restoration of injured nerves is an incredible task. The fabrication of an ideal nerve guide that fulfills the requirement to regenerate nerve tissue is a herculean challenge requiring a combination of both biochemical and topographical cues. The present study explores the combinatorial effect of aligned nanofibers and the regulated delivery of triiodothyronine and retinoic acid on nerve regeneration. A sequential release mechanism is adopted in fabricating the nanofiber scaffold, with triiodothyronine incorporated into the nanofiber shell ensuring its prior release, followed by retinoic acid (entrapped within zein nanoparticles) from the core. The composite nanofibers thus fabricated possess excellent mechanical, physical and thermal properties and good topographical morphology and were highly biocompatible. The nanofibers were scrutinized for their efficacy in stimulating differentiation to a neuronal phenotype. The elongation factor (E-factor) of the neural cells had doubled in the bioactive incorporated composite compared to other scaffolds, as observed on phalloidin staining of their cytoskeleton, which endorsed enhanced neural differentiation on the fabricated nanofiber scaffold. There was a significant increase in the expression of neural-lineage specific markers on investigation of mRNA by real time PCR, showing a 10 fold increase in the gene expression of β-III-tubulin, a 5.5 fold increase for microtubule associated protein 2 gene and 3.5 fold for neurofilament M gene in the cells cultured over bioactive incorporated aligned nanofiber composites. Similarly protein expression was analyzed by immunofluorescence and flow cytometry studies, which showed an increase in the expression of β-III-tubulin in the composite nanofiber. This corroborates that neuronal differentiation is enhanced by the aligned nanotopography and spatio-temporal delivery of triiodothyronine and retinoic acid, opening avenues for nerve regenerative graft fabrication.

Nerve injuries are often debilitating as its regeneration occurs in a slow and laborious manner. Remediation of nerve injury is a colossal task as functional restoration in larger gaps seldom occurs due to the complex nerve regeneration mechanism. A nanofiber‐based graft material has been fabricated to provide topographical and biochemical cues to encourage neural differentiation. Laminin plays a crucial role in supporting peripheral nerve regeneration and hence aligned polyvinyl cinnamate nanofibers surface‐conjugated with laminin‐derived cell‐adhesion peptides have been fabricated to improve selective neural adhesion and regeneration. Further, triiodothyronine has been encapsulated within the nanofibers enabling its sustained release so as to bolster regeneration and reinstate the lost functionality to the damaged nerve. The fabricated nanofibers were characterized for its physicochemical, morphological, and topographical properties. Nanofibers were biocompatible, improved cell adhesion rate, and illustrated favourable interaction with cells. Gene expression (showed 9.5 and 4.1 fold increase in β‐tubulin and MAP 2 expression, respectively) and protein expression (immunofluorescence, flow cytometry, and western blot) studies confirmed the positive influence of the scaffold over cell differentiation. The studies were extrapolated to adult zebrafish model with a surgical incision in posterior lateral line. The biocomposite treated group showed earlier functional restoration of the nerve compared with control groups detected by touch‐evoked response. Thus, the combination of aligned nanofibers providing topographical cue, along with the peptides and triiodothyronine serving as biochemical cues, has a robust potential to restore functionality to the injured nerve, thereby opening avenues for fabrication of regenerative nerve grafts.

With rising incidents of non-healing chronic wounds, functional restoration of the organs after a major wound insult is an essential requisite. The delivery of endogenous molecules through nanomaterials for supporting healing of such wounds has gained impetus in the past decade. Triiodothyronine (T 3 ) is a hormone that exerts its activity at various target organs and is reported to play a critical role in repair and regeneration of tissues after injury. The encapsulation of T 3 in nanofibers and its sustained release to enable wound healing has been attempted for the first time in this work. The physico-chemical characterization confirmed the encapsulation and uniform distribution of the hormone in the nanofiber. Functional characterization of the composite nanofibers revealed the significant positive influence of the T 3 -entrapped nanofibers on the proliferation and migration of skin cells. Thus this work reveals the effect of prolonged sustained delivery of T 3 from nanofibers which might promote the healing of chronic wounds.