Purpose: The aim of this in vivo study is to compare the variation in implant stability quotient (ISQ) values of implants using resonance frequency analysis (RFA) to access the immediately loaded (within 24 h) implants at different time intervals using Penguin RFA system. Materials and Methods: Ten Nobel Replace Tapered RP implants were placed in posterior mandible region of patients. Patients were included in the study after fulfilling the inclusion criteria. Prior to enrolling in the study, thorough information about the procedure was provided to the patients, and written consent was obtained for the same. Immediate loading was done within 24 h using tooth-colored self-cure acrylic resin. Penguin RFA System was used to measure the ISQ of dental implants. The ISQ values were recorded immediately after implant placement, after 4 weeks, after 8 weeks, and after 12 weeks. Results: The mean ISQ values of the immediately loaded implants at the time of placement were 82.60, after 4 weeks were 80.60, after 8 weeks were 83.70, and after 12 weeks were 87.10. There was a statistically significant difference between the ISQ values at the time of placement of implants and after 12 weeks. Conclusion: This study concluded that immediate loading in posterior mandible single tooth region is a safe and predictable option.