Aim of the study: Determination of liver enzyme [AST(GOT),ALT(GPT),ALK] and evaluate the performance of defferent JAK2 V617F mutation PCR:PCR-ARMS,PCR-RFLP,Real Time-PCR .The patients and methods of work collected samples from the Center for Hematology and Oncology in Kirkuk City , the study included ( 88) patients whose ages ranged between (2-79) and also included ( 40) of natural persons as control cases whose ages ranged between (7-63). In molecular tests, ( 75) a patient sample of the total patient samples was used. The Determination of liver enzyme (AST,ALT,ALK) was performed using several equipment provided by the company (randoxs and Biomerieux) for the studied enzyme, Real-time examination was done using a kit equipped by (Bioneer-Korea) company. Rresults: the results showed the increase in liver enzymes in patients (male and female) compared to (male and female) control at the level of significance. As for the results of molecular tests, the results showed that using the assay (RFLP) that 22 samples gave genotype (GG) and 53 samples gave genotype (GT), as for the assay (ARMS), all samples gave the genotype (GT) Conclusions :High liver enzymes in leukemia patients.

CYP17 gene is the key in the metabolic pathway of sexual steroids, it codes for two enzymes 17–alpha hydroxylase and 17, 20 lyase who play an important role in the production of sex steroid hormones. The current study included study of the relationship of this gene with PCOS women in reproductive age. Blood samples were collected from two groups of women: first group consists of 98 women infected with polycystic ovaries syndrome, the second consists of 25 healthy women to detect the presence of mutation within the gene, then calculate allele frequency of mutant and wild allele for this gene, then repeat genotypes in infected women and compared with healthy once, were calculated body mass index BMI of two groups and measure the level of sex hormones (LH, FSH, Prolactin, Testosterone) in both groups, were the results showed significant differences between patients and healthy women in BMI and the level of hormones (LH, Prolactine) while there is no significant differences in the (FSH, Testosterone) hormone. Three pattern of genotype were obtained: TT, homozygous wild, (TC) much lower than the frequency in the healthy 0.82 while the frequency of allele C among patients 0.44, which is more than double than in healthy 0.18. The current results showed absence CC genotype in healthy women and appeared only in women with PCOS. This monitor the presence of link between this gene and PCOS as may be considered the mutation -34 (T C) in the promoter of gene CYP17 marker of the disease.

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