Experiments with 14C-labeled glucose indicated that the orange-colored variety of Streptococcus bovis assimilated about 12 % of consumed glucose in the synthetic medium and 6 % in the peptone-yeast extract medium. These values are very much higher than those of ordinary lactic streptococci which show the values of around 0.4%. Although the yield of lactic acid did not exceed 90 % of consumed glucose, this species is also a homofermenter if the assimilated glucose is taken into account. Different from ordinary lactic acid bacteria, S. bovis can grow in the medium containing ammonium salt as a sole source of nitrogen but organic nitrogen is preferable even for this species.The characteristics of orange-colored cocci isolated from bovine rumen were described by 01 et al. (1-3). The cocci were determined to be a variety of Streptococcus bovis Orla-Jensen, for reason of many similarities except for the pigmentation. They also reported that the lactic acid recovery from fermented glucose by this organism was much less than the theoretical value of homo-lactic fermentation.The pigmentation developed in nutritionally poor medium, and growth yield was markedly higher than other homo-lactic fermenters. The orange coloration in S. bovis had already been noticed by HUNGATE (4) but his description on this point was very slight. This paper describes the glucose metabolism of the orange-colored S. bovis. MATERIALS AND METHODS.

No abstract

The fermentation of various pentoses by lactic acid bacteria was first described by Fred, Peterson and Anderson, and the following formula was proposed for the reaction: C5H1005-~ CH3CHOH000H + CH3000H (I) This formula has, heretofore, been admitted by many investigators. According to the formula equimolar quantities of lactic and acetic acids are formed from pentose, namely, the theoretical yield of lactic acid is 60% by weight of the consumed pentose. By an isotopic experiment using Lactobacillus sp. (2), (3), (4) it has been established that the methyl and carboxyl groups of acetate arise from C-1 and C-2, while-000H,-CHOH and-CH3 groups of lactate are derived from C-3, C-4 and C-5 of pentose molecule respectively.

No abstract

The anaerobic brick-colored cocci were known previously to be similar to Strepto coccus bovis Orla-Jensen in fermenting starch and producing L (+) lactic acid, but their taxonomic position was remained unsolved. In this paper the cocci were compared with type cultures of Sc. bovis including ATCC 9809 and IFO 12058. They were coincided well in morphological characters, fermentable sugars, relatively low lactic acid recovery, utilization of inorganic nitrogen and other physiological properties except for: Present strains are strict anaerobic and generally more or less colored.