A new formulation of macro aggregated albumin (MAA) to be labeled with 99mTc was prepared by a simple and fast method. The suspension of macroagreggated albumin was prepared by diluting 0.4ml human serum albumin (200mg/ml) with normal saline (sterilized and pyrogen free) Concentratedhydrochloric acid was added to denature the protein and this step was followed by the addition of tin in the form of stannite . 3.5ml of polyethylene glycol(PEG) (1mg/ml) was also added to act as antiagglomerating agent. The pH was adjusted to 5.2 with NaOH and the suspension was heated with continuousstirring to get aggregation The vials contents were allowed to react with 99mTc and high radiochemical purity was obtained (greater than 95%) after 15min. 99mTc-MAA was stable for at least six hours.The organ distribution data in mice showed that more than 93.0% of the injected dose has accumulated in the lungs with a negligible amount of radioactivity to be detected in the non target organs.The size distribution data showed that 80.0% of the particles occur in the range of 10—80μm. The freeze-dried preparation of Sn-MAA kits (sterile and pyrogen free) were stable for at least 170 day.

99mTc-dimercapto Succinic acid freeze-dried kit(99mTc-DMSA) was prepared by reaction of kit that is contained (1mg of 2.3 meso dimercaptosuccinic acid,0.336 mg of stannous chloride, 40mg insitol and 0.9 ascorbic acid as anti oxidant) with Na99mTcO4 at pH equal to (2-4) that is the optimal condition to formation 99mTc-DMSA complex.The radiochemical purity and stability of 99mTc-DMSA in liquid and dried forms were determined on gel filtration column scanning “GCS”. The labeling yield of complex formation was greater than 99% after 15 min. from addition of Na99mTcO4. The complex in liquid form was stable during ten hours while in lyophilized form six months.The biodisribution of kit was studied on white male mice that the results indicate to the ratio of dose concentration accumulated into kidneys is greater than29% of injected dose, however, little amounts of complex was detected in non target organs.

The new preparation of colloidal Tin phosphate labeled with technisum- 99m for bone marrow studies. The method was applied with heating sodium bihydrogen phosphate with carboxy methyl cellulose (CMC) as stabilizer in neutral medium then cooed at room temperature, followed by adding stannouschloride as stannite. The chemical analysis indicates that the radiochemical purity is greater than 99% with suitable particle size distribution.The results of biodistribution indicate that the preparative agent was accumulated with higher concentration in bone marrow reach to 30% of total injected dose and negligible percent in non target organs. The results meaning that the new preparative agent is a good agent for using as bone marrow scintigraphy compared with others.