Bacterial cellulose (BC) is a type of biopolymer produced as primary metabolism product by many genera of bacteria such as Acetobacter, Rhizobium, Agrobacterium, Psuedomonas and Sarcina. This article aimed to study the effect of some factors on cellulose production from Rhizobium leguminosarum bv. viciae which isolated from root nodules of Vicia faba plant collected from Abu-Ghraib in Baghdad. Some environmental and nutritional factors were examined including : carbon and nitrogen sources, pH, temperature and incubation period. Results showed that maximum production of cellulose was gained by Hestrin-Schram medium (HS medium) containing 2% fructose as carbon source, 1% yeast extract as nitrogen source, as well as optimum pH, temperature, and incubation period were (5, 30°C for 7 days) respectively .Cellulose dry weight was analyzed by Fourier transform infrared spectroscopy (FTIR) technique and the results showed the presence of the functional groups of bacterial cellulose.

Fructan is a kind of biopolymer produced from plants, fungi, yeast and bacteria. It is classified as prebiotics food and enhanced the growth of probiotics bacterial strains which improves the health of the individual. This article aimed to study the production and extraction of fructan from Pseudomonas putida isolated from food samples and study the effect of fructan on the viable cell number of Lactobacillus acidophilus. The results showed that Pseudomonas putida have the ability to produce fructan when cultured on production medium (YPS), fructan exhibited as white powder when extracted from YPS and the amount of fructan was 3.6 mg/100 ml, also the results showed that fructan can increase the viable cell number of Lactobacillus acidophilus when added to MRS. FTIR analysis of fructan produced from Pseudomonas putida revealed that the functional groups found in chemical structure of fructan, which it was (C-O, C-H, O-H and C=O).