Strawberry wilt is an established disease of strawberry crops caused by fungus Fusarium solani. In Mexico, strawberry cultivation represents an important productive activity for several rural areas; however, wilt disease affects producers economically. The objectives of this research were: (a) to identify and morphologically characterize strain “MA-FC120” associated with root rot and wilting of strawberry crops in Santa Cruz Analco, municipality of San Salvador el Verde, Puebla-Mexico; (b) to evaluate the potential of single and multiple applications of four broad-spectrum fungicides used against F. solani in vitro. Plant tissue samples were collected from strawberry crops in Puebla-Mexico with presence of symptoms of desiccation and root rot. Strain “MA-FC120” was identified as F. solani, being the causal agent of wilt and root rot in strawberry plants from Santa Cruz Analco. Fungicide Benomyl 50® showed the highest percentage of inhibition on F. solani (100%) under in vitro conditions. The fungicide Mancosol 80® and Talonil 75® at low concentration (600 and 450 mg L−1) showed no toxicity, being harmless to strain MA-FC120. However, fungicide Talonil 75® showed slight toxicity at the dose recommended by the manufacturer and moderate toxicity in high concentration (1350 mg L−1). Likewise, Captan 50® in its three concentrations evaluated showed slight toxicity, obtaining around 50% on the classification scale established by International Organization for Biological Control (IOBC).