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The aim of the present study was to determine the levels of ochratoxin A (OTA) in pigs experimentally exposed to this mycotoxin and to evaluate if bile may be used to assess exposure to OTA. Twelve hybrid pigs were divided into 3 equal groups, a control group D0, and 2 experimental groups, D1 fed with 50 µg OTA/kg diet, and D2 fed with 500 µg OTA/kg diet for 15 days. At the end of the test, the animals were euthanized and samples of different tissues and biological fluids were analysed by HPLC-fluorescence detection for the presence of OTA. Samples of unconventional edible tissues such as lung and heart were also taken for analysis because they are used in typical Italian regional dishes. The Italian guidance value for OTA of 1 µg/kg established for pork meat and derived products has been exceeded in all the matrices from both the experimental groups. The comparison between OTA levels detected in D1 and D2 groups showed clearly a linear dose-response relationship. Based on the mean values measured, OTA distribution follows the order blood plasma > lung > kidney (in D1 group), heart (in D2 group) > heart (in D1 group), kidney (in D2 group) > bile > liver > fat > muscle. Analysis of bile can be useful for the detection of OTA in pigs. However, since blood can easily be taken from pigs, and given the correlation between the mycotoxin concentration detected in this matrix and the concentrations detected in the others, OTA level in blood is a more viable approach to assessing the presence of OTA in edible tissues. As lung and heart may contain high concentrations of OTA, the analytical controls should also include these matrices.

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A total of 172 different salamis were purchased from farms and small salami factories located in four Italian regions (Piedmont, Veneto, Calabria and Sicily), and analysed for the presence of ochratoxin A (OTA). Analysis were performed by high-performance liquid chromatography coupled to a fluorimetric detector (HPLC-FLD). The detection limit (LOD) for the method used was 0.05 µg/kg, while the quantitation limit (LOQ) was 0.20 µg/kg; the average recovery rate was 89.1%. OTA was detected in 22 salamis, and 3 samples exceeded the Italian guidance value for OTA in pork meat (1 µg/kg). In particular, what emerges from this research is the high percentage of spicy salamis among positive samples (68.2%, 15 out of 22), although spicy salamis are only 27.3% of the total number of samples collected and analysed. Red chili pepper contaminated by OTA could be responsible for the presence of the mycotoxin in these spicy salamis. It follow that, also the control of some ingredients used in the manufacture of these meat products, like spices, should not be neglected.