Background: Cell sheet technique using mesenchymal stem cells is a high-level strategy in periodontal regenerative medicine. Although recent studies have shown the role of MSCSs in increased dental supporting tissues and bone, there is not a systematic review focused specifically on assessing periodontal regeneration in orthotopic animal models. Objective: To evaluate the potential of mesenchymal stem cell sheets (MSCSs) on periodontal regeneration, compared to control, in experimental animal models. Methods: Pre-clinical studies in periodontal defects of animal models were considered eligible. The electronic search included the MEDLINE, Web of Science, EMBASE and LILACS databases. The review was conducted according to the Preferred Reporting Item for Systematic Reviews and Meta-Analyses statement guidelines. Results: A total of 17 of the 3989 studies obtained from the electronic database search were included. MSCSs included dental follicle (DF) MSCSs, periodontal ligament (PL) MSCSs, dental pulp (DP) MSCSs, bone marrow (BM) MSCSs, alveolar periosteal (AP) MSCSs and gingival (G) MSCSs. Regarding cell sheet inducing protocol, most of the studies used ascorbic acid (58.82%). Others used culture dishes grafted with a temperature-responsive polymer (41.18%). Adverse effects were not identified in the majority of studies. Meta-analysis was not considered because of methodological heterogeneities. PDL-MSCSs demonstrated to be superior for periodontal regeneration enhancement compared to control, but in an induced inflammatory microenvironment, DF-MSCSs were better. Moreover, DF-MSCSs, DP-MSCSs, and BM-MSCSs showed improved results compared to control. Conclusion: MSCSs can improve periodontal regeneration in animal periodontal defect models.
Protease-activated receptor-2 (PAR2) is associated with the pathogenesis of many chronic diseases with inflammatory characteristics, including periodontitis. This study aimed to evaluate how the activation of PAR2 can affect the osteogenic activity of human periodontal ligament stem cells (PDLSCs) in vitro. PDLSCs collected from three subjects were treated in osteogenic medium for 2, 7, 14, and 21 days with trypsin (0.1 U/mL), PAR2 specific agonist peptide (SLIGRL-NH2) (100 nM), and PAR2 antagonist peptide (FSLLRY-NH2) (100 nM). Gene (RT-qPCR) expression and protein expression (ELISA) of osteogenic factors, bone metabolism, and inflammatory cytokines, cell proliferation, alkaline phosphatase (ALP) activity, alizarin red S staining, and supernatant concentration were assessed. Statistical analysis of the results with a significance level of 5% was performed. Activation of PAR2 led to decreases in cell proliferation and calcium deposition (p < 0.05), calcium concentration (p < 0.05), and ALP activity (p < 0.05). Additionally, PAR2 activation increased gene and protein expression of receptor activator of nuclear factor kappa-Β ligand (RANKL) (p < 0.05) and significantly decreased the gene and protein expression of osteoprotegerin (p <0. 05). Considering the findings, the present study demonstrated PAR2 activation was able to decrease cell proliferation, decreased osteogenic activity of PDLSCs, and upregulated conditions for bone resorption. PAR2 may be considered a promising target in periodontal regenerative procedures.
Dedico esta disertación a mi familia: Mis amados padres, Miguel Ángel y Clelia Elisa, mi ejemplo a seguir, mi mayor amor, mi fuente de valores, mi puerto más seguro, mi camino, motor y motivo.Mi hermano Miguel Ángel, mi primer y mejor amigo de vida, por siempre cuidar de mí, ser mi modelo a seguir, mi soporte, mi apoyo y tener siempre el mejor de los consejos preparado para mí en el momento más oportuno.Mi hermano Kevin Arthur, mi amigo más confiable, que a pesar de su corta edad nos enseña tanto sobre la vida. Su madurez e inteligencia emocional fueron fundamentales para mí en los momentos más difíciles lejos de la familia.A mi Carla, por el gran apoyo, compañerismo, cariño, amor e incentivo durante este proceso importante en mi crecimento profesional y personal.A mi bebé Miguel Angel, nuestra bendición que está em camino y mi motivación diaria a seguir adelante.Mi cuñada Lady Diana, la hermana que siempre quise y tengo la suerte de tener en mi familia, además me dió la bendición de tener a mi sobrina Samantha.A mi sobrina Samantha, el tenerla en casa es uma verdadera bendición de Dios. Gracias por iluminar nuestro hogar.A mi abuela Isabel Castillo, mi consejera más experta, cada palabra de ella siempre queda grabada em mi mente y mi corazón.A mis tíos Rafael y Cristina, quienes fueron como mis padres aquí en Brasil, les estaré eternamente agradecido por todo el apoyo y la estabilidad que me brindaron en todo momento.A mis ángeles, Hortencia Guevara, Miguel Huamán y Napoleón Espejo, sé que desde el cielo siguen guiando mis pasos e iluminándome.Soy muy agradecido a todos ustedes por entender y apoyar este logro, que no solo es mío sino también de ustedes. ¡Gracias por esta conquista! AGRADECIMENTOS Agradeço a Deus porque sem ele nada disso seria possível, por me colocar as pessoas certas para chegar até aqui.Agradeço a minha orientadora Profa. Dra. Marinella Holzhausen Caldeira pela confiança, mesmo antes de me conhecer pessoalmente, por me oferecer a oportunidade de seguir meus sonhos e ter a possibilidade de estudar no Brasil e principalmente na FOUSP, por me incentivar sempre a crescer tanto como profissional quanto como ser humano, me inspirando a seguir em todo momento para frente, sonhando, mas sempre com os pés no chão; por ser uma orientadora presente, exemplo de professora e como uma mãe na pós-graduação para mim e para todos seus orientandos. Muito obrigado por tanto, prezada professora Marinella! Agradeço aos professores dos quais tive a honra de aprender e conviver, oferecendo sempre o melhor deles para estimular meu aprendizado Prof. Dr.
Objetivo: Identificar los principales factores asociados a la recesión de la papila interdental de incisivos centrales superiores. Material y métodos: Se analizó una muestra no probabilística de 86 pacientes de la sección de Periodoncia e Implantes del Departamento de Estomatología del Hospital Central de la Fuerza Aérea del Perú. El tipo de estudio fue observacional, correlacional simple, transversal y prospectivo. Se evaluaron clínicamente factores como forma coronal dentaria y biotipo gingival; y radiográficamente distancias de la unión cemento esmalte proximal al punto de contacto interdentario (UCEp-PC), punta de la papila al punto de contacto interdentario (PP-PC), cresta ósea al punto de contacto interdentario (CO-PC), cresta ósea a la unión cemento esmalte proximal (CO-UCEp), ancho interdental (AI), ancho de la cresta ósea (AC) y ancho de la punta de la papila (APP). Resultados: En todos los pacientes de estudio, el nivel de recesión, CO-PC y APP fueron predictores individuales significativos (p
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